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Volume 28, Number 3—March 2022

Higher Viral Stability and Ethanol Resistance of Avian Influenza A(H5N1) Virus on Human Skin

Risa Bandou, Ryohei HiroseComments to Author , Takaaki Nakaya, Hajime Miyazaki, Naoto Watanabe, Takuma Yoshida, Tomo Daidoji, Yoshito Itoh, and Hiroshi Ikegaya
Author affiliation: Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan

Main Article

Table 6

Survival time and half-lives of various recombinant viruses on the surface of human skin*

Median survival time (95% CI), h‡
Median half-life (95% CI), h‡
4 (log10 FFU)
3 (log10 FFU)
2 (log10 FFU)
rH5N1-H5N3-NA 2.04 (1.79–2.31) 0.09 (0.08–0.10) 0.12 (0.10–0.14) 0.18 (0.15–0.21)
rH5N1-H5N3-HA 2.06 (1.77–2.37) 0.09 (0.08–0.11) 0.12 (0.10–0.14) 0.18 (0.15–0.21)
rH5N3-H5N1-NA 4.65 (3.94–5.43) 0.20 (0.17–0.25) 0.27 (0.23–0.33) 0.41 (0.35–0.49)
rH5N3-H5N1-NS 2.18 (1.83–2.55) 0.10 (0.08–0.12) 0.13 (0.11–0.16) 0.19 (0.16–0.23)
rH5N3-H5N1-M 2.22 (1.87–2.61) 0.10 (0.08–0.12) 0.13 (0.11–0.16) 0.19 (0.16–0.24)
rH5N3-H5N1-HA 2.16 (1.83–2.52) 0.09 (0.07–0.11) 0.13 (0.11–0.15) 0.19 (0.16–0.23)

*FFU, focus-forming unit. †A/crow/Kyoto/53/04 (H5N1) recombined with the NA and HA genes of A/Duck/Hong Kong/820/80 (H5N3) are defined as rH5N1-H5N3-NA and rH5N1-H5N3-HA, respectively. In addition, A/Duck/Hong Kong/820/80 (H5N3) recombined into the NA, NS, M, and HA genes of A/crow/Kyoto/53/04 (H5N1) was defined as rH5N3-H5N1-NA, rH5N3-H5N1-NS, rH5N3-H5N1-M, rH5N3-H5N1-HA. ‡The elapsed time was used as the explanatory variable (x-axis), and the logarithmic virus titer was used as the explained variable (y-axis). A linear regression analysis with logarithmic link function was performed for each virus to create a curve of regression (Appendix Figure 4). Since the detection limit of each influenza virus titer was 101 FFUs, the X value (when the Y value of the regression curve was 1.0) was used as the survival time. §The half-life of each virus was calculated from the slope of each regression curve when the amount of virus remaining on the surface was 2, 3, or 4 log10 FFUs.

Main Article

Page created: January 23, 2022
Page updated: February 21, 2022
Page reviewed: February 21, 2022
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