Retrospective Genomic Characterization of a 2017 Dengue Virus Outbreak, Burkina Faso
Andrew G. Letizia
1, Catherine B. Pratt
1 , Michael R. Wiley, Anne T. Fox, Mba Mosore, Bright Agbodzi, Clara Yeboah, Selassie Kumordjie, Nicholas Di Paola, Kone Cisse Assana, David Coulidiaty, Casimir Ouedraogo, Joseph H. Kofi Bonney, William Ampofo, Zékiba Tarnagda, and Lassana Sangaré
Author affiliations: Naval Medical Research Unit TWO, Singapore (A.G. Letizia); University of Nebraska Medical Center, Omaha, Nebraska, USA (C.B. Pratt, M.R. Wiley); Naval Medical Research Unit THREE, Ghana Detachment, Accra, Ghana (A.T. Fox); Noguchi Memorial Institute for Medical Research, Accra (M. Mosore, B. Agbodzi, C. Yeboah, S. Kumordjie, J.H.K. Bonney, W. Ampofo); US Army Medical Research Institute of Infectious Disease, Frederick, Maryland, USA (N. Di Paola); Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso, Burkina Faso (K.C. Assana, Z. Tarnagda); Centre Hospitalier Universitaire Yalgado Ouédraogo, Ouagadougou, Burkina Faso (D. Coulidiaty, C. Ouedraogo, L. Sangaré)
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Figure 5
Figure 5. Nucleotide identity between dengue virus molecular diagnostics and all sequenced DENV genomes from the 2017 Burkina Faso dengue outbreak. The map indicates the proportion of genomes from each country with >1 mismatches against the Trioplex PCR forward primer (A), probe (B), and reverse1 primer (C). Countries in gray have no data. DENV-1 and DENV-3 have concordant nucleotide identity to the primers and probe, but most DENV-2 forward primer and reverse1 primer in sequences from Africa have a high proportion of genomes with >1 mismatches to the Trioplex PCR’s primers and probe. DENV-1, dengue virus serotype 1; DENV-2, dengue virus serotype 2; DENV-3, dengue virus serotype 3
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