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Volume 28, Number 7—July 2022
Dispatch

Use of Human Intestinal Enteroids to Evaluate Persistence of Infectious Human Norovirus in Seawater

Marion Desdouits1Comments to Author , David Polo12, Cecile Le Mennec, Sofia Strubbia3, Xi-Lei Zeng, Khalil Ettayebi, Robert L. Atmar, Mary K. Estes, and Françoise S. Le Guyader
Author affiliations: Institut Franҫais de Recherche pour l’Exploitation de la Mer, Nantes, France (M. Desdouits, D. Polo, C. Le Mennec, S. Strubbia, F.S. Le Guyader); Baylor College of Medicine, Houston, Texas, USA (X.-L. Zeng, K. Ettayebi, R.L. Atmar, M.K. Estes)

Main Article

Figure 1

Study design on use of HIEs to evaluate persistence of infectious human norovirus in seawater. Comparison of the stability of 2 human norovirus strains (GII.3 indicated by green, GII.4 indicated by blue) and TuV (orange) in seawater. We conducted 3 independent experiments with different fresh seawater samples. Spiked seawater (120 mL) was split in 10 mL aliquots in glass tubes, incubated at 12°C in the dark under constant rotation (10 rpm), and randomly sampled once or twice per week for 5 weeks (35 days). Grey arrows indicate steps or treatments applied to all samples; blue-green arrows indicate steps or treatments applied to human norovirus and control without virus; orange arrows indicate steps or treatments applied to TuV only. HIE, human intestinal enteroid, NoV, norovirus; qRT-PCR, one-step quantitative reverse transcription PCR; TCID50, 50% median tissue culture infectious dose; TuV, Tulane virus.

Figure 1. Study design on use of HIEs to evaluate persistence of infectious human norovirus in seawater. Comparison of the stability of 2 human norovirus strains (GII.3 indicated by green, GII.4 indicated by blue) and TuV (orange) in seawater. We conducted 3 independent experiments with different fresh seawater samples. Spiked seawater (120 mL) was split in 10 mL aliquots in glass tubes, incubated at 12°C in the dark under constant rotation (10 rpm), and randomly sampled once or twice per week for 5 weeks (35 days). Grey arrows indicate steps or treatments applied to all samples; blue-green arrows indicate steps or treatments applied to human norovirus and control without virus; orange arrows indicate steps or treatments applied to TuV only. HIE, human intestinal enteroid, NoV, norovirus; qRT-PCR, one-step quantitative reverse transcription PCR; TCID50, 50% median tissue culture infectious dose; TuV, Tulane virus.

Main Article

1These first authors contributed equally to this article.

2Current affiliation: Centro de Investigaciones Biologicas–Facultade de Bioloxía & CRETUS, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.

3Current affiliation: SECALIM UMR 104 Oniris/Inrae, Nantes, France.

Page created: May 18, 2022
Page updated: June 18, 2022
Page reviewed: June 18, 2022
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