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Volume 28, Number 9—September 2022
Research

Rapid Adaptation of Established High-Throughput Molecular Testing Infrastructure for Monkeypox Virus Detection

Dominik Nörz, Hui Ting Tang, Petra Emmerich, Katja Giersch, Nicole Fischer, Stephan Schmiedel, Marylyn M. Addo, Martin Aepfelbacher, Susanne Pfefferle, and Marc LütgehetmannComments to Author 
Author affiliations: University Medical Center Hamburg-Eppendorf, Hamburg, Germany (D. Nörz, H.T. Tang, K. Giersch, N. Fischer, S. Schmiedel, M.M. Addo, M. Aepelbacher, S. Pfefferle, M. Lütgehetmann); Bernhard-Nocht Institute for Tropical Medicine, Hamburg (P. Emmerich, M.M. Addo, S. Pfefferle); German Center for Infection Research, Hamburg (M.M. Addo)

Main Article

Figure 1

Linearity data for the dual-target monkeypox virus assay rapidly adapted from established high-throughput molecular testing infrastructure. A) Nonvariola orthopoxvirus target; B) monkeypox virus target; C) absolute Ct for nonvariola orthopox virus target; D) absolute Ct for monkeypox virus target. Linearity was determined by serial dilution of monkeypox virus reference material from cell culture supernatant of Congo Basin monkeypox strain collected in 1987. Analysis was performed on Validation Manager software (Finbiosoft, https://finbiosoft.com). Nonvariola orthopoxvirus slope was −3.52, r2 = 0.999; monkeypox virus slope was −3.40, r2 = 0.999. Ct, cycle threshold.

Figure 1. Linearity data for the dual-target monkeypox virus assay rapidly adapted from established high-throughput molecular testing infrastructure. A) Nonvariola orthopoxvirus target; B) monkeypox virus target; C) absolute Ct for nonvariola orthopox virus target; D) absolute Ct for monkeypox virus target. Linearity was determined by serial dilution of monkeypox virus reference material from cell culture supernatant of Congo Basin monkeypox strain collected in 1987. Analysis was performed on Validation Manager software (Finbiosoft, https://finbiosoft.com). Nonvariola orthopoxvirus slope was −3.52, r2 = 0.999; monkeypox virus slope was −3.40, r2 = 0.999. Ct, cycle threshold.

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Page created: July 13, 2022
Page updated: August 19, 2022
Page reviewed: August 19, 2022
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