Figure 2. Amplification curves of clinical samples, including internal controls for dual-target monkeypox virus assay rapidly adapted from established high-throughput molecular testing infrastructure. A) Nonvariola orthopoxvirus; B) monkeypox virus; C) internal control. Samples included clinical swab specimens of monkeypox lesions, oropharyngeal swab samples, and EDTA plasma from patients with confirmed monkeypox, Hamburg, Germany. Asterisk (*) in panel B indicates the positive control curve in channel 2, which was the cell culture supernatant of Congo Basin monkeypox strain collected in 1987. West Africa strain samples exhibit a reduction of approximately one third in relative fluorescence increase for monkeypox virus, due to a known mismatch in the probe region (Appendix Figure 1).