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Volume 28, Number 9—September 2022

Rapid Adaptation of Established High-Throughput Molecular Testing Infrastructure for Monkeypox Virus Detection

Dominik Nörz, Hui Ting Tang, Petra Emmerich, Katja Giersch, Nicole Fischer, Stephan Schmiedel, Marylyn M. Addo, Martin Aepfelbacher, Susanne Pfefferle, and Marc LütgehetmannComments to Author 
Author affiliations: University Medical Center Hamburg-Eppendorf, Hamburg, Germany (D. Nörz, H.T. Tang, K. Giersch, N. Fischer, S. Schmiedel, M.M. Addo, M. Aepelbacher, S. Pfefferle, M. Lütgehetmann); Bernhard-Nocht Institute for Tropical Medicine, Hamburg (P. Emmerich, M.M. Addo, S. Pfefferle); German Center for Infection Research, Hamburg (M.M. Addo)

Main Article

Table 2

Software settings for run protocol for a dual-target MPXV assay rapidly adapted from established high-throughput molecular testing infrastructure*

Protocol setting
Channel setting, use
1, Not used
2, Monkeypox
3, Nonvariola
4, Not used
5, Internal control
Relative fluorescence increase
PCR cycling conditions UNG incubation Pre-PCR step 1st measurement 2nd measurement Cooling
No. cycles Predefined 1 5 45 Predefined
No. steps Predefined 3 2 2 Predefined
Temperature Predefined 55°C; 60°C; 65°C 95°C; 55°C 91°C; 58°C Predefined
Hold time Predefined 120 s; 360 s; 240 s 5 s; 30 s 5 s; 25 s Predefined
Data acquisition Predefined None End of each cycle End of each cycle Predefined

*Protocol was run on 400 µL swab samples. Protocol adapted for cobas omni Utility Channel (Roche Diagnostics, Relative fluorescence increase thresholds were used for automated result calls. NA, not applicable; UNG, uracyl-N-glycosylase.

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Page created: July 13, 2022
Page updated: August 19, 2022
Page reviewed: August 19, 2022
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