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Volume 28, Number 9—September 2022

Rapid Adaptation of Established High-Throughput Molecular Testing Infrastructure for Monkeypox Virus Detection

Dominik Nörz, Hui Ting Tang, Petra Emmerich, Katja Giersch, Nicole Fischer, Stephan Schmiedel, Marylyn M. Addo, Martin Aepfelbacher, Susanne Pfefferle, and Marc LütgehetmannComments to Author 
Author affiliations: University Medical Center Hamburg-Eppendorf, Hamburg, Germany (D. Nörz, H.T. Tang, K. Giersch, N. Fischer, S. Schmiedel, M.M. Addo, M. Aepelbacher, S. Pfefferle, M. Lütgehetmann); Bernhard-Nocht Institute for Tropical Medicine, Hamburg (P. Emmerich, M.M. Addo, S. Pfefferle); German Center for Infection Research, Hamburg (M.M. Addo)

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Table 3

Hit rates during limit of detection studies of dual-target MPXV assay rapidly adapted from established high-throughput molecular testing infrastructure*

Concentration, copies/mL NVAR MPXV Overall
100 21/21 21/21 21/21
50 21/21 21/21 21/21
25 21/21 21/21 21/21
12.5 20/21 21/21 21/21
6.25 19/21 20/21 21/21
3.125 11/21 13/21 15/21
1.56 11/21 11/21 17/21
0.78 3/21 6/21 7/21

*Results represent no. positive/no. tested. Limits of detection were determined by serial dilution of a quantified MPXV standard (quantified by digital PCR) as a reference. Concentrations represent copies/mL of specimen. Dilution series were generated automatically using a STARlet Liquid Handler (Hamilton, We calculated 95% probability of detection by using MedCalc statistical software ( MPXV, monkeypox virus; NVAR, nonvariola orthopoxvirus.

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Page created: July 13, 2022
Page updated: August 19, 2022
Page reviewed: August 19, 2022
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