Volume 29, Number 3—March 2023
Dispatch
Mycobacterium leprae in Armadillo Tissues from Museum Collections, United States
Daniel Romero-Alvarez
, Daniel Garzon-Chavez, Mary Jackson, Charlotte Avanzi, and A. Townsend Peterson
, Daniel Garzon-Chavez, Mary Jackson, Charlotte Avanzi, and A. Townsend Peterson
Table 2
Characteristics of armadillo tissue samples from US museums identified as positive by standard PCR for Mycobacterium leprae*
| Voucher/tissue no. | Sample ID | Tissue type | Preservation (%) | DNA con. | Country | State | Sex | Year | Type | Subtype | Ct |
|---|---|---|---|---|---|---|---|---|---|---|---|
| YPM 16952 | 63 | Muscle | Ethanol (95) | 20 | USA | Texas | F | 2014 | ND | ND | ND |
| YPM 15982 | 66 | Muscle | Ethanol (95) | 13 | USA | Texas | F | 2015 | ND | ND | ND |
| YPM 15294 | 80 | Muscle | Ethanol (95) | 2.89 | USA | Texas | F | 2013 | 3 | 3I | 34.41 |
| YPM 16954 | 95 | Muscle | Ethanol (95) | 11 | USA | Texas | M | 2014 | ND | ND | ND |
| YPM 15295 | 97 | Muscle | Ethanol (95) | 14 | USA | Texas | F | 2013 | ND | ND | ND |
| YPM 15292 | 99 | Muscle | Ethanol (95) | 5.7 | USA | Texas | F | 2013 | ND | ND | ND |
| YPM 15296 | 103 | Muscle | Ethanol (95) | 8.9 | USA | Texas | F | 2013 | ND | ND | ND |
| YPM 15293 | 105 | Muscle | Ethanol (95) | 4.76 | USA | Texas | M | 2013 | ND | ND | ND |
| YPM 14944 | 109 | Muscle | Ethanol (95) | 9.3 | USA | Texas | NA | 2014 | 3 | 3I | 23.15 |
| YPM 15315 | 110 | Muscle | Ethanol (95) | 0.0041 | USA | Texas | F | 2013 | ND | ND | ND |
| YPM 15298 | 111 | Muscle | Ethanol (95) | 27 | USA | Texas | F | 2013 | ND | ND | ND |
| YPM 15299 | 115 | Muscle | Ethanol (95) | 43 | USA | Texas | F | 2012 | ND | ND | ND |
| UAM 46589 | 118 | Liver | DMSO | 11 | Paraguay | Canindeyu | F | 1996 | ND | ND | ND |
| MSB 140243 | 138 | Liver | Ethanol (95) | 37 | Bolivia | Beni | NA | 1993 | ND | ND | ND |
| TTU 75235 | 158 | Spleen | Frozen | 19 | USA | Texas | F | 1996 | 3 or 4 | ND | 35.12 |
| TTU 82457 | 194 | Muscle | Frozen | 3.82 | USA | Texas | M | 2000 | 3 | 3I | 31.58 |
| TTU 75360 | 209 | Spleen | Frozen | 20 | USA | Texas | F | 1996 | 3 | 3I | 25.83 |
| TTU 80673 | 212 | Spleen | Frozen | 31 | USA | Texas | M | 1999 | ND | ND | ND |
*We identified a total of 18 M. leprae-positive samples. Bold text indicates samples suitable for whole-genome sequencing (n = 2). Samples negative for subtyping were determined unsuitable for whole-genome sequencing. Ct determined by quantitative PCR. Ct, cycle threshold; DNA con., concentration of total DNA per sample; NA, no data available; ND, not determined
Page created: January 12, 2023
Page updated: February 20, 2023
Page reviewed: February 20, 2023
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.