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Volume 3, Number 4—December 1997


THEME ISSUE
Foodborne
Controlling Emerging Foodborne Microbial Hazards

Epidemiology and Detection as Options for Control of Viral and Parasitic Foodborne Disease

Lee-Ann JaykusComments to Author 
Author affiliation: North Carolina State University, Raleigh, North Carolina, USA

Main Article

Table 2

Emerging detection methods for human enteric viruses in foods

Field
Pathogen Sample Detection limit app. Ref.
Nucleic Acid Extraction/RT-PCR
HAV Clams 2000 particles/g
(10 PFU/g) No 44
Poliovirus Oysters 38 PFU/20 g
(2 PFU/g) No 42
HAV Clams/Oysters 100 PFU/1.5 g
(67 PFU/g) No 43
Norwalk Clams/Oysters 5-10 PCRU/1.5 g
(3-7 PCRU/g) No 43
Poliovirus Oysters/Mussels 10 PFU/5 g
(2 PFU/g) No 45
SRSV Oysters/Mussels Not specified Yes 46
Polivirus,
HAV Clams 100 PFU/50 g
(2 PFU/g) No 50
Norwalk Clams 1000 PCRU/50 g
(20 PCRU/g) No 50
Norwalk Various 20-200 PCRU/10 g
(2-20 PCRU/g) No 47
Antibody-Capture/RT-PCR
HAV Clams/Oysters Not specified No 48
HAV Oysters Not specified Yes 19
Virion Concentration
Poliovirus,
HAV Oysters 10 PFU/50 g
(0.02 PFU/g) Yes 49,
Norwalk Oysters 4500 PCRU/50 g
(90 PCRU/g) No 49,51
Poliovirus,
HAV Clams 1000 PFU/50 g
(20 PFU/g) No 50
Norwalk Clams 100 PCRU/50 g
(2 PCRU/g) No 50

HAV = hepatitis A virus; RT-PCR = reverse transcriptase polymerase chain reaction; PCRU = PCR-amplifiable units; SRSV = small round-structured gastrointestinal virus.

Main Article

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The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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