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Volume 30, Number 8—August 2024
Research

Metagenomic Detection of Bacterial Zoonotic Pathogens among Febrile Patients, Tanzania, 2007–20091

Robert J. Rolfe, Sarah W. Sheldon, Luke C. Kingry, Jeannine M. Petersen, Venance P. Maro, Grace D. Kinabo, Wilbrod Saganda, Michael J. Maze, Jo E.B. Halliday, William L. Nicholson, Renee L. Galloway, Matthew P. Rubach, and John A. CrumpComments to Author 
Author affiliations: Duke University Department of Medicine Division of Infectious Diseases and International Health, Durham, North Carolina, USA (R.J. Rolfe, M.P. Rubach, J.A. Crump); Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (S.W. Sheldon, L.C. Kingry, J.M. Petersen); Kilimanjaro Christian Medical Centre, Moshi, Tanzania (V.P. Maro, G.D. Kinabo, M.P. Rubach, J.A. Crump); Kilimanjaro Christian Medical University College, Moshi (V.P. Maro, G.D. Kinabo, J.A. Crump); Mawenzi Regional Referral Hospital, Moshi (W. Saganda); University of Otago Department of Medicine, Christchurch, New Zealand (M.J. Maze); University of Glasgow School of Biodiversity, One Health and Veterinary Medicine, Glasgow, Scotland, UK (J.E.B. Halliday); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (W.L. Nicholson, R.L. Galloway); Duke-National University of Singapore Programme in Emerging Infectious Diseases, Singapore (M.P. Rubach); Duke University Global Health Institute, Durham (M.P. Rubach, J.A. Crump); Centre for International Health, University of Otago, Dunedin, New Zealand (J.A. Crump)

Main Article

Figure 1

Phylogenetic tree for Candidatus Neoehrlichia spp. identified during metagenomic detection of bacterial zoonotic pathogens among febrile patients, Tanzania, 2007–2009. Bold text indicates the sequence from this study. Numbers in parentheses indicate GenBank accession numbers. A 1,467-bp 16S sequence amplified from a bone marrow aspirate from a patient from South Africa (GenBank accession no. OP208838) matched 100% over the 296-bp variable regions 1 and 2 target sequence amplified in this study (18). Scale bar indicates nucleotide substitutions per site.

Figure 1. Phylogenetic tree for Candidatus Neoehrlichia spp. identified during metagenomic detection of bacterial zoonotic pathogens among febrile patients, Tanzania, 2007–2009. Bold text indicates the sequence from this study. Numbers in parentheses indicate GenBank accession numbers. A 1,467-bp 16S sequence amplified from a bone marrow aspirate from a patient from South Africa (GenBank accession no. OP208838) matched 100% over the 296-bp variable regions 1 and 2 target sequence amplified in this study (18). Scale bar indicates nucleotide substitutions per site.

Main Article

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Main Article

1Preliminary results of this study were presented at the 72nd American Society of Tropical Medicine and Hygiene Annual Meeting; October 18­–22, 2023; Chicago, Illinois, USA.

Page created: June 15, 2024
Page updated: July 20, 2024
Page reviewed: July 20, 2024
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