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Volume 30, Number 8—August 2024
Research

Metagenomic Detection of Bacterial Zoonotic Pathogens among Febrile Patients, Tanzania, 2007–20091

Robert J. Rolfe, Sarah W. Sheldon, Luke C. Kingry, Jeannine M. Petersen, Venance P. Maro, Grace D. Kinabo, Wilbrod Saganda, Michael J. Maze, Jo E.B. Halliday, William L. Nicholson, Renee L. Galloway, Matthew P. Rubach, and John A. CrumpComments to Author 
Author affiliations: Duke University Department of Medicine Division of Infectious Diseases and International Health, Durham, North Carolina, USA (R.J. Rolfe, M.P. Rubach, J.A. Crump); Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (S.W. Sheldon, L.C. Kingry, J.M. Petersen); Kilimanjaro Christian Medical Centre, Moshi, Tanzania (V.P. Maro, G.D. Kinabo, M.P. Rubach, J.A. Crump); Kilimanjaro Christian Medical University College, Moshi (V.P. Maro, G.D. Kinabo, J.A. Crump); Mawenzi Regional Referral Hospital, Moshi (W. Saganda); University of Otago Department of Medicine, Christchurch, New Zealand (M.J. Maze); University of Glasgow School of Biodiversity, One Health and Veterinary Medicine, Glasgow, Scotland, UK (J.E.B. Halliday); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (W.L. Nicholson, R.L. Galloway); Duke-National University of Singapore Programme in Emerging Infectious Diseases, Singapore (M.P. Rubach); Duke University Global Health Institute, Durham (M.P. Rubach, J.A. Crump); Centre for International Health, University of Otago, Dunedin, New Zealand (J.A. Crump)

Main Article

Figure 3

Phylogenetic tree of Leptospira sequences detected in metagenomic analysis of bacterial zoonotic pathogens among febrile patients, Tanzania, 2007–2009. The tree compares sequences from the 16S V1–V2 of the L. kerchnerii and L. borgpetersenii cohort from this study (bold text) to sequences from closely related Leptospira species. Numbers in parentheses indicate GenBank accession numbers. Scale bar indicates nucleotide subsitutions per site.

Figure 3. Phylogenetic tree of Leptospira sequences detected in metagenomic analysis of bacterial zoonotic pathogens among febrile patients, Tanzania, 2007–2009. The tree compares sequences from the 16S V1–V2 of the L. kerchnerii and L. borgpetersenii cohort from this study (bold text) to sequences from closely related Leptospira species. Numbers in parentheses indicate GenBank accession numbers. Scale bar indicates nucleotide subsitutions per site.

Main Article

1Preliminary results of this study were presented at the 72nd American Society of Tropical Medicine and Hygiene Annual Meeting; October 18­–22, 2023; Chicago, Illinois, USA.

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