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Volume 30, Number 9—September 2024
Research

Formation of Single-Species and Multispecies Biofilm by Isolates from Septic Transfusion Reactions in Platelet Bag Model

Cheryl Anne Hapip, Erin Fischer, Tamar Perla Feldman, and Bethany L. BrownComments to Author 
Author affiliation: American Red Cross, Rockville, Maryland, USA

Main Article

Figure 2

Effects of different growth media (TSBG) versus APH PLT for most bacteria on biofilm CFU recovery (A) and CV biofilm formation (B) on platelet bag coupons after 24 hours of incubation at 35°C. Baseline readings of TSBG and APH PLT without bacteria were subtracted from the readings at OD550. Leclercia adecarboxylata and the polymicrobial biofilms containing L. adecarboxylata showed a significant (p<0.05) increase in CFU within the biofilm and CV biomass when grown in APH PLT compared with TSBG. Acinetobacter spp. recovered from the biofilm was significantly decreased when grown in APH PLT. Each dot represents an individual biologic replicate inoculated with the corresponding monomicrobial or polymicrobial bacterial species. A, Acinetobacter spp.; APH PLT, apheresis platelets; AU, absorbance unit; CV, crystal violet; L, L. adecarboxylata; NS, not significant; OD, optical density; pos, positive control (Staphylococcus epidermidis); S, S. saprophyticus; TSBG, tryptic soy broth–glucose.

Figure 2. Effects of different growth media (TSBG) versus APH PLT for most bacteria on biofilm CFU recovery (A) and CV biofilm formation (B) on platelet bag coupons after 24 hours of incubation at 35°C. Baseline readings of TSBG and APH PLT without bacteria were subtracted from the readings at OD550. Leclercia adecarboxylata and the polymicrobial biofilms containing L. adecarboxylata showed a significant (p<0.05) increase in CFU within the biofilm and CV biomass when grown in APH PLT compared with TSBG. Acinetobacter spp. recovered from the biofilm was significantly decreased when grown in APH PLT. Each dot represents an individual biologic replicate inoculated with the corresponding monomicrobial or polymicrobial bacterial species. A, Acinetobacter spp.; APH PLT, apheresis platelets; AU, absorbance unit; CV, crystal violet; L, L. adecarboxylata; NS, not significant; OD, optical density; pos, positive control (Staphylococcus epidermidis); S, S. saprophyticus; TSBG, tryptic soy broth–glucose.

Main Article

Page created: July 16, 2024
Page updated: August 20, 2024
Page reviewed: August 20, 2024
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