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Volume 30, Number 9—September 2024
Research

Formation of Single-Species and Multispecies Biofilm by Isolates from Septic Transfusion Reactions in Platelet Bag Model

Cheryl Anne Hapip, Erin Fischer, Tamar Perla Feldman, and Bethany L. BrownComments to Author 
Author affiliation: American Red Cross, Rockville, Maryland, USA

Main Article

Figure 4

Average total CFU (n = 3) percentage comparison for 4 combinations of Leclercia adecarboxylata, Acinetobacter spp., and Staphylococcus saprophyticus mixed in equal parts and incubated together. Each bacterial species was measured in each polymicrobial biofilm after 24-hour incubation in apheresis platelets at both 35°C and room temperature. A) L. adecarboxylata and Acinetobacter spp.; B) L. adecarboxylata and S. saprophyticus; C) Acinetobacter spp. and S. saprophyticus; D) all 3 species incubated together. Even with a longer doubling time, L. adecarboxylata outcompeted Acinetobacter spp. in CFU percentage, whereas S. saprophyticus only accounts for 2% CFU in L–A–S polymicrobial biofilm grown at room temperature. Error bars represent SDs of the replicates. A, Acinetobacter spp.; L, L. adecarboxylata; RT, room temperature; S, S. saprophyticus.

Figure 4. Average total CFU (n = 3) percentage comparison for 4 combinations of Leclercia adecarboxylata, Acinetobacter spp., and Staphylococcus saprophyticus mixed in equal parts and incubated together. Each bacterial species was measured in each polymicrobial biofilm after 24-hour incubation in apheresis platelets at both 35°C and room temperature. A) L. adecarboxylata and Acinetobacter spp.; B) L. adecarboxylata and S. saprophyticus; C) Acinetobacter spp. and S. saprophyticus; D) all 3 species incubated together. Even with a longer doubling time, L. adecarboxylata outcompeted Acinetobacter spp. in CFU percentage, whereas S. saprophyticus only accounts for 2% CFU in L–A–S polymicrobial biofilm grown at room temperature. Error bars represent SDs of the replicates. A, Acinetobacter spp.; L, L. adecarboxylata; RT, room temperature; S, S. saprophyticus.

Main Article

Page created: July 16, 2024
Page updated: August 20, 2024
Page reviewed: August 20, 2024
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