Volume 30, Number 9—September 2024
Research
Formation of Single-Species and Multispecies Biofilm by Isolates from Septic Transfusion Reactions in Platelet Bag Model
Figure 1
![Overview of procedural steps taken to evaluate monomicrobial and polymicrobial biofilms grown on platelet bag coupons in different media (apheresis platelets vs. TSB–glucose) and at 22°C vs. 35°C. A) Study methodology for measuring biofilm production through crystal violet assay. B) Methods for determining CFU per coupon and comparative species composition of polymicrobial biofilms. CV, crystal violet; OD, optical density; PAS, platelet additive solution; RT, room temperature; TSA, tryptic soy agar; TSB, tryptic soy broth.](/eid/images/24-0372-F1.jpg)
Figure 1. Overview of procedural steps taken to evaluate monomicrobial and polymicrobial biofilms grown on platelet bag coupons in different media (apheresis platelets vs. TSB–glucose) and at 22°C vs. 35°C. A) Study methodology for measuring biofilm production through crystal violet assay. B) Methods for determining CFU per coupon and comparative species composition of polymicrobial biofilms. CV, crystal violet; OD, optical density; PAS, platelet additive solution; RT, room temperature; TSA, tryptic soy agar; TSB, tryptic soy broth.
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Page updated: August 20, 2024
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