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Volume 30, Number 9—September 2024
Research

Formation of Single-Species and Multispecies Biofilm by Isolates from Septic Transfusion Reactions in Platelet Bag Model

Cheryl Anne Hapip, Erin Fischer, Tamar Perla Feldman, and Bethany L. BrownComments to Author 
Author affiliation: American Red Cross, Rockville, Maryland, USA

Main Article

Figure 1

Overview of procedural steps taken to evaluate monomicrobial and polymicrobial biofilms grown on platelet bag coupons in different media (apheresis platelets vs. TSB–glucose) and at 22°C vs. 35°C. A) Study methodology for measuring biofilm production through crystal violet assay. B) Methods for determining CFU per coupon and comparative species composition of polymicrobial biofilms. CV, crystal violet; OD, optical density; PAS, platelet additive solution; RT, room temperature; TSA, tryptic soy agar; TSB, tryptic soy broth.

Figure 1. Overview of procedural steps taken to evaluate monomicrobial and polymicrobial biofilms grown on platelet bag coupons in different media (apheresis platelets vs. TSB–glucose) and at 22°C vs. 35°C. A) Study methodology for measuring biofilm production through crystal violet assay. B) Methods for determining CFU per coupon and comparative species composition of polymicrobial biofilms. CV, crystal violet; OD, optical density; PAS, platelet additive solution; RT, room temperature; TSA, tryptic soy agar; TSB, tryptic soy broth.

Main Article

Page created: July 16, 2024
Page updated: August 20, 2024
Page reviewed: August 20, 2024
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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