Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link

Disclaimer: Early release articles are not considered as final versions. Any changes will be reflected in the online version in the month the article is officially released.

Volume 30, Number 9—September 2024
Dispatch

Avian and Human Influenza A Virus Receptors in Bovine Mammary Gland

Charlotte KristensenComments to Author , Henrik E. Jensen, Ramona Trebbien, Richard J. Webby, and Lars E. Larsen
Author affiliations: University of Copenhagen, Frederiksberg, Denmark (C. Kristensen, H.E. Jensen, L.E. Larsen); Statens Serum Institut, Copenhagen, Denmark (R. Trebbien); St. Jude Children’s Research Hospital, Memphis, Tennessee, USA (R.J. Webby)

Main Article

Figure 2

Results of staining showing wide expression of the duck receptor for influenza A virus (designated MAA-II) in the alveoli of the bovine mammary gland. A) An example of the MAA-II staining of a mammary gland from a cow in late lactation. Arrowhead indicates expression of the duck receptor in active alveoli. Asterisks indicate less staining in the less active alveoli. Original magnification ×10. B, C) MAA-II staining of a duct (B) and an alveolus (C) in a 3-year-old cow. Original magnification ×60. D, E) Positive staining obtained from the image analysis. Green line shows the region of interest. Original magnification ×60. F) A neuraminidase pretreatment negative control showed markedly reduced staining of the MAA-II lectin in the alveolus but some nonspecific staining in the duct epithelium. The staining was visualized using Vector Blue (Vector Laboratories, https://vectorlabs.com). Original magnification ×60.

Figure 2. Results of staining showing wide expression of the duck receptor for influenza A virus (designated MAA-II) in the alveoli of the bovine mammary gland. A) An example of the MAA-II staining of a mammary gland from a cow in late lactation. Arrowhead indicates expression of the duck receptor in active alveoli. Asterisks indicate less staining in the less active alveoli. Original magnification ×10. B, C) MAA-II staining of a duct (B) and an alveolus (C) in a 3-year-old cow. Original magnification ×60. D, E) Positive staining obtained from the image analysis. Green line shows the region of interest. Original magnification ×60. F) A neuraminidase pretreatment negative control showed markedly reduced staining of the MAA-II lectin in the alveolus but some nonspecific staining in the duct epithelium. The staining was visualized using Vector Blue (Vector Laboratories, https://vectorlabs.com). Original magnification ×60.

Main Article

Page created: July 17, 2024
Page updated: August 09, 2024
Page reviewed: August 09, 2024
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external