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Volume 30, Number 9—September 2024
Dispatch

Avian and Human Influenza A Virus Receptors in Bovine Mammary Gland

Charlotte KristensenComments to Author , Henrik E. Jensen, Ramona Trebbien, Richard J. Webby, and Lars E. Larsen
Author affiliations: University of Copenhagen, Frederiksberg, Denmark (C. Kristensen, H.E. Jensen, L.E. Larsen); Statens Serum Institut, Copenhagen, Denmark (R. Trebbien); St. Jude Children’s Research Hospital, Memphis, Tennessee, USA (R.J. Webby)

Main Article

Figure 1

Results of staining showing wide expression of the human receptor for influenza A virus (designated SNA) in the bovine mammary gland. A) An example of the SNA staining of a mammary gland from a cow in late lactation. Arrowhead indicates expression of the human receptor in the active alveoli. Asterisks indicate less staining in the less active alveoli. Original magnification ×10. B, C) SNA staining of a duct (B) and (C) an alveolus in a 7-year-old cow. Original magnification ×60. D, E) Positive staining obtained from the image analysis. Green line shows the region of interest. Original magnification ×60. F) A neuraminidase pretreatment negative control showed markedly reduced staining of the SNA lectin. Original magnification ×60. The staining was visualized using Vector Blue (Vector Laboratories, https://vectorlabs.com).

Figure 1. Results of staining showing wide expression of the human receptor for influenza A virus (designated SNA) in the bovine mammary gland. A) An example of the SNA staining of a mammary gland from a cow in late lactation. Arrowhead indicates expression of the human receptor in the active alveoli. Asterisks indicate less staining in the less active alveoli. Original magnification ×10. B, C) SNA staining of a duct (B) and (C) an alveolus in a 7-year-old cow. Original magnification ×60. D, E) Positive staining obtained from the image analysis. Green line shows the region of interest. Original magnification ×60. F) A neuraminidase pretreatment negative control showed markedly reduced staining of the SNA lectin. Original magnification ×60. The staining was visualized using Vector Blue (Vector Laboratories, https://vectorlabs.com).

Main Article

Page created: July 17, 2024
Page updated: August 21, 2024
Page reviewed: August 21, 2024
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