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Volume 31, Number 10—October 2025

Dispatch

Emergence of Bordetella holmesii–Associated Pertussis-Like Illness, Northern India, 2019–2023

Nishant Shekhar, Deepa Sharma, Surajit Chakraborty, Rajesh Kumar, Rajneesh Singh Rawat, Nirmal Kaundal, Pawan Kumar, Tigran Avagyan, Deeksha Chauhan, Neha Jain, Megha Sharma, Arun Kumar, and Vikas GautamComments to Author 
Author affiliation: Post Graduate Institute of Medical Education and Research, Chandigarh, India (N. Shekhar, R. Kumar, R.S. Rawat, D. Chauhan, V. Gautam); World Health Organization Country Office India, New Delhi, India (D. Sharma, N. Kaundal, T. Avagyan); University of Miami Miller School of Medicine, Miami, Florida, USA (S. Chakraborty); India Ministry of Health and Family Welfare, New Delhi (P. Kumar); Yatharth Hospital, Noida, India (N. Jain); All India Institute of Medical Science−Bilaspur, Bilaspur, India (M. Sharma)

Main Article

Table 1

Results of real-time quantitative PCR using the IS481, ptxS1, and hIS1001 genes to differentiate Bordetella species in study of emergence of Bordetella holmesii–associated pertussis-like illness, northern India, 2019–2023

Category No. samples
IS481
Positive for Bordetella by multiplex PCR 197
Monoplex PCR, ptxS1
Positive for B. pertussis 52
Negative
 145
pIS1001
Positive for Bordetella by multiplex PCR 17
Monoplex PCR, ptxS1
Positive for B. parapertussis 2
Negative
 15
IS481 and hIS1001
Positive for Bordetella by multiplex PCR 143
Monoplex PCR, ptxS1
Positive for B. pertussis and B. holmesii 15
Negative for B. holmesii
 128
IS481 and pIS1001
Multiplex PCR positive 26
Monoplex PCR, ptxS1
Positive for B. pertussis and B. parapertussis 7
Negative
 19
Total 778

Main Article

Page created: September 02, 2025
Page updated: September 25, 2025
Page reviewed: September 25, 2025
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