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Volume 31, Number 11—November 2025

Research

Monkeypox Virus Partial-Genome Amplicon Sequencing for Improvement of Genomic Surveillance during Mpox Outbreaks

Jiusheng Deng, Daisy McGrath, Kimberly Wilkins, Luis A. Haddock, Whitni Davidson, Demi B. Rabeneck, Joseph Madden, Vaughn Wicker, Adrienne Amuri-Aziza, Tony Wawina-Bokalanga, Placide Mbala-Kingebeni, Christina L. Hutson, Yu Li, and Crystal GiganteComments to Author 
Author affiliation: Chenega Enterprise Systems and Solutions, LLC, Chesapeake, Virginia, USA (J. Deng); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (J. Deng, D. McGrath, K. Wilkins, L.A. Haddock, W. Davidson, D.B. Rabeneck, J. Madden, V. Wicker, C.L. Hutson, Y. Li, C. Gigante); University of Kinshasa, Kinshasa, Democratic Republic of the Congo (A. Amuri-Aziza, T. Wawina-Bokalanga, P. Mbala-Kingebeni)

Main Article

Figure 4

Amplicon-based phylogenetic assignment of monkeypox virus by 10-kb and 15-kb regions in Nextclade 3.10.0 (https://clades.nextstrain.org) for study of partial-genome amplicon sequencing for improvement of genomic surveillance during mpox outbreaks. Sixteen clinical specimens from Democratic Republic of the Congo were assigned to clade Ia or Ib, and 33 clinical specimens from United States were assigned to clade IIb lineage B.1.

Figure 4. Amplicon-based phylogenetic assignment of monkeypox virus by 10-kb and 15-kb regions in Nextclade 3.10.0 (https://clades.nextstrain.org) for study of partial-genome amplicon sequencing for improvement of genomic surveillance during mpox outbreaks. Sixteen clinical specimens from Democratic Republic of the Congo were assigned to clade Ia or Ib, and 33 clinical specimens from United States were assigned to clade IIb lineage B.1.

Main Article

Page created: November 18, 2025
Page updated: December 10, 2025
Page reviewed: December 10, 2025
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