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Volume 31, Number 11—November 2025

Research

Monkeypox Virus Partial-Genome Amplicon Sequencing for Improvement of Genomic Surveillance during Mpox Outbreaks

Jiusheng Deng, Daisy McGrath, Kimberly Wilkins, Luis A. Haddock, Whitni Davidson, Demi B. Rabeneck, Joseph Madden, Vaughn Wicker, Adrienne Amuri-Aziza, Tony Wawina-Bokalanga, Placide Mbala-Kingebeni, Christina L. Hutson, Yu Li, and Crystal GiganteComments to Author 
Author affiliation: Chenega Enterprise Systems and Solutions, LLC, Chesapeake, Virginia, USA (J. Deng); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (J. Deng, D. McGrath, K. Wilkins, L.A. Haddock, W. Davidson, D.B. Rabeneck, J. Madden, V. Wicker, C.L. Hutson, Y. Li, C. Gigante); University of Kinshasa, Kinshasa, Democratic Republic of the Congo (A. Amuri-Aziza, T. Wawina-Bokalanga, P. Mbala-Kingebeni)

Main Article

Table 4

Genetic variants over the 10-kb and 15-kb genomic regions of MPXV in 36 clinical specimens for study of partial-genome amplicon sequencing for improvement of genomic surveillance during mpox outbreaks*

Specimen no. Country sSNP nsSNP Total SNPs† Indels Variants‡ SNP type§ SNPs
1–20 USA 15 27 42 2 44 GA>AA 17
Other
 25
21–30 DRC 90 17 107 20 127 GA>AA 22
Other
 85
31–36 DRC 60 10 70 7 77 GA>AA 23
Other 47

*See Table 1. DRC, Democratic Republic of the Congo; indels, insertions and deletions; MPXV, monkeypox virus; nsSNP: nonsynonymous SNP; SNP, single-nucleotide polymorphism; sSNP, synonymous SNP. USA, United States. †Total number of SNPs include sSNP and nsSNPs. ‡Variants include total number of SNPs plus indels. §SNP type GA>AA (5′–3′) includes TC>TT (5′–3′).

Main Article

Page created: November 18, 2025
Page updated: December 10, 2025
Page reviewed: December 10, 2025
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