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Volume 31, Number 11—November 2025

Research

Monkeypox Virus Partial-Genome Amplicon Sequencing for Improvement of Genomic Surveillance during Mpox Outbreaks

Jiusheng Deng, Daisy McGrath, Kimberly Wilkins, Luis A. Haddock, Whitni Davidson, Demi B. Rabeneck, Joseph Madden, Vaughn Wicker, Adrienne Amuri-Aziza, Tony Wawina-Bokalanga, Placide Mbala-Kingebeni, Christina L. Hutson, Yu Li, and Crystal GiganteComments to Author 
Author affiliation: Chenega Enterprise Systems and Solutions, LLC, Chesapeake, Virginia, USA (J. Deng); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (J. Deng, D. McGrath, K. Wilkins, L.A. Haddock, W. Davidson, D.B. Rabeneck, J. Madden, V. Wicker, C.L. Hutson, Y. Li, C. Gigante); University of Kinshasa, Kinshasa, Democratic Republic of the Congo (A. Amuri-Aziza, T. Wawina-Bokalanga, P. Mbala-Kingebeni)

Main Article

Figure 3

Conserved SNPs over the 10-kb and the 15-kb regions identified in silico among respective clades from 88 published moneypox virus genomes for study of partial-genome amplicon sequencing for improvement of genomic surveillance during mpox outbreaks. A) Unique SNPs over the 10-kb region. B) Unique SNPs over the 15-kb region. Large black points represent SNPs. Vertical shaded bars represent the binding sites of the amplicon primers. OPG, orthopoxvirus gene; SNP, single-nucleotide polymorphism.

Figure 3. Conserved SNPs over the 10-kb and the 15-kb regions identified in silico among respective clades from 88 published moneypox virus genomes for study of partial-genome amplicon sequencing for improvement of genomic surveillance during mpox outbreaks. A) Unique SNPs over the 10-kb region. B) Unique SNPs over the 15-kb region. Large black points represent SNPs. Vertical shaded bars represent the binding sites of the amplicon primers. OPG, orthopoxvirus gene; SNP, single-nucleotide polymorphism.

Main Article

Page created: November 18, 2025
Page updated: December 10, 2025
Page reviewed: December 10, 2025
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