Metatranscriptomic Identification of Trubanaman Virus Sequences in Patient with Encephalitis, Australia
Krispin Hajkowicz
, John Woodford, Elango Subramonia Pillai, Andrea Henden, Kym Lowry, Mary E. Petrone, Patrick N.A. Harris, and Edward C. Holmes
Author affiliation: Royal Brisbane and Women’s Hospital, Brisbane, Queensland, Australia (K. Hajkowicz, E. Subramonia Pillai, A. Henden, P.N.A. Harris); University of Queensland Centre for Clinical Research, Brisbane (K. Hajkowicz, K. Lowry, P.N.A. Harris); The School of Medical Sciences, University of Sydney, Sydney, New South Wales, Australia (K. Hajkowicz, M.E. Petrone, E.C. Holmes); Ipswich Hospital, Ipswich, Queensland, Australia (J. Woodford); Queensland Institute of Medical Research-Berghofer, Brisbane (J. Woodford, A. Henden); Queensland Paediatric Infectious Diseases Sakzewski Laboratory, Brisbane (K. Lowry); Pathology Queensland, Brisbane (P.N.A. Harris)
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Figure
Figure. Phylogenetic tree of orthobunyavirus M segment sequences from this study and previously collected mosquito samples in study of metatranscriptomic identification of Trubanaman virus in patient with encephalitis, Australia. Bold font indicates the human sequence from this study; other sequences are from mosquito orthobunyaviruses previously identified in Australia. Sequences were aligned using MAFFT (https://mafft.cbrc.jp/alignment/server/index.html). The phylogeny was estimated using the maximum-likelihood approach in PhyML (http://atgc.lirmm.fr/phyml), by using the general time reversible model of nucleotide substitution and gamma-distributed rate variation among sites. Bootstrap support values are displayed at nodes. Scale bar indicates nucleotide substitutions per site.
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