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Volume 31, Number 7—July 2025

Dispatch

Multisystemic Disease and Septicemia Caused by Presumptive Burkholderia pseudomallei in American Quarter Horse, Florida, USA

Jason J. ThorntonComments to Author , John F. Roberts, David P. AuCoin, and Apichai Tuanyok
Author affiliation: University of Florida College of Veterinary Medicine, Gainesville, Florida, USA (J.J. Thornton, J.F. Roberts, A. Tuanyok); University of Nevada School of Medicine, Reno, Nevada, USA (D.P. AuCoin)

Main Article

Figure 3

Immunohistochemical examination of tissues from American quarter horse with multisystemic disease and septicemia caused by presumptive Burkholderia pseudomallei, Florida, USA. Photomicrographs show immunohistochemistry for monoclonal antibodies 3A2 (A), 4C7 (B), 4C4 (C), and 5B4 (D) of the submandibular lymph node. A) Intra-phagocytic bacilli demonstrated strong immunoreactivity to 3A2 monoclonal antibody to B. pseudomallei lipopolysaccharides (LPS) O-Ag B/B2 (black arrows). Original magnification ×100. B) Intra-phagocytic bacilli were diffusely immunonegative for 4C7 monoclonal antibody to B. pseudomallei LPS O-ag serotype A. Original magnification ×40. C) Intra-phagocytic bacilli demonstrated weak multifocal immunoreactivity to 4C4 monoclonal antibody to B. pseudomallei capsular polysaccharides (black arrows). Original magnification ×100. D) Intra-phagocytic bacilli demonstrated strong immunoreactivity to 5B4 monoclonal antibody to B. pseudomallei LPS O-Ag B/B2 (black arrows). Original magnification ×100. Bacilli were immunonegative to 3D11 (not shown) monoclonal antibody to Bm LPS O-Ag.

Figure 3. Immunohistochemical examination of tissues from American quarter horse with multisystemic disease and septicemia caused by presumptive Burkholderia pseudomallei, Florida, USA. Photomicrographs show immunohistochemistry for monoclonal antibodies 3A2 (A), 4C7 (B), 4C4 (C), and 5B4 (D) of the submandibular lymph node. A) Intra-phagocytic bacilli demonstrated strong immunoreactivity to 3A2 monoclonal antibody to B. pseudomallei lipopolysaccharides (LPS) O-Ag B/B2 (black arrows). Original magnification ×100. B) Intra-phagocytic bacilli were diffusely immunonegative for 4C7 monoclonal antibody to B. pseudomallei LPS O-ag serotype A. Original magnification ×40. C) Intra-phagocytic bacilli demonstrated weak multifocal immunoreactivity to 4C4 monoclonal antibody to B. pseudomallei capsular polysaccharides (black arrows). Original magnification ×100. D) Intra-phagocytic bacilli demonstrated strong immunoreactivity to 5B4 monoclonal antibody to B. pseudomallei LPS O-Ag B/B2 (black arrows). Original magnification ×100. Bacilli were immunonegative to 3D11 (not shown) monoclonal antibody to Bm LPS O-Ag.

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Page created: May 01, 2025
Page updated: June 17, 2025
Page reviewed: June 17, 2025
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