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Volume 32, Supplement –Summer 2026

SUPPLEMENT ISSUE
Supplement

Laboratory Assessment of Wastewater as Surveillance Tool for West Nile Virus, United States

Jessica Spring, Audrey Long, Emily Davis, Shannon R. Matzinger, Allison Wheeler, Shelby L. Lyons, Daniel L. Jacobs, Rory Welsh, Jeffrey W. Mercante, Aaron C. Brault, and Holly R. HughesComments to Author 
Author affiliation: Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (J. Spring, A. Long, E. Davis, S.L. Lyons, D.L. Jacobs, A.C. Brault, H.R. Hughes); Colorado Department of Public Health and Environment, Denver, Colorado, USA (S.R. Matzinger, A. Wheeler); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (R. Welsh, J.W. Mercante)

Main Article

Figure 1

West Nile virus (WNV) RNA detection by digital reverse transcription PCR in spiked wastewater samples up to 72 hours from laboratory assessment of wastewater as surveillance tool for WNV, United States. WNV RNA recovery over time from wastewater spiked with a high concentration of WNV (known yield 106–107 RNA copies) (A) or a low concentration of WNV (known yield 104–105 RNA copies) (B). Data are represented as averages from multiple experiments; error bars represent standard SD.

Figure 1. West Nile virus (WNV) RNA detection by digital reverse transcription PCR in spiked wastewater samples up to 72 hours from laboratory assessment of wastewater as surveillance tool for WNV, United States. WNV RNA recovery over time from wastewater spiked with a high concentration of WNV (known yield 106–107 RNA copies) (A) or a low concentration of WNV (known yield 104–105 RNA copies) (B). Data are represented as averages from multiple experiments; error bars represent standard SD.

Main Article

Page created: August 20, 2026
Page updated: September 10, 2026
Page reviewed: September 10, 2026
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