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Volume 32, Number 4—April 2026

Research

Enhanced Detection of Coccidioides spp. Fungi from Environmental Samples Using Droplet Digital PCR

Jessica Paulette Segovia-Mota, Ricardo Eaton-González, Jimena Carrillo-Tripp, and Meritxell RiquelmeComments to Author 
Author affiliation: Centro de Investigación Científica y Educación Superior de Ensenada (CICESE), Ensenada, Mexico (J.P. Segovia-Mota, J. Carrillo-Tripp, M. Riquelme); Facultad de Ciencias Marinas, Universidad Autónoma de Baja California, Ensenada (R. Eaton-González)

Main Article

Figure 2

Nested PCR detection of Coccidioides spp. fungi DNA from environmental samples using droplet digital PCR, Baja California, Mexico. Agarose gel electrophoresis (2%) stained with ethidium bromide shows the results from nested PCR amplification of Coccidioides spp. internal transcribed spacer region from 20 DNA extracted from soil samples collected in RG, Baja California. Positive bands are ≈120 bp. Lanes: M, GeneRuler ladder (100-bp); RG1–RG20, DNA from soil samples; C+, positive control of 0.48 ng of C. posadasii DNA used as template; C–, negative control of highly refined Type I ultrapure (HPLC) water. RG, Rancho Gilbert.

Figure 2. Nested PCR detection of Coccidioides spp. fungi DNA from environmental samples using droplet digital PCR, Baja California, Mexico. Agarose gel electrophoresis (2%) stained with ethidium bromide shows the results from nested PCR amplification of Coccidioides spp. internal transcribed spacer region from 20 DNA extracted from soil samples collected in RG, Baja California. Positive bands are ≈120 bp. Lanes: M, GeneRuler ladder (100-bp); RG1–RG20, DNA from soil samples; C+, positive control of 0.48 ng of C. posadasii DNA used as template; C–, negative control of highly refined Type I ultrapure (HPLC) water. RG, Rancho Gilbert.

Main Article

Page created: February 20, 2026
Page updated: April 15, 2026
Page reviewed: April 15, 2026
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