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Volume 32, Number 4—April 2026

Research

Enhanced Detection of Coccidioides spp. Fungi from Environmental Samples Using Droplet Digital PCR

Jessica Paulette Segovia-Mota, Ricardo Eaton-González, Jimena Carrillo-Tripp, and Meritxell RiquelmeComments to Author 
Author affiliation: Centro de Investigación Científica y Educación Superior de Ensenada (CICESE), Ensenada, Mexico (J.P. Segovia-Mota, J. Carrillo-Tripp, M. Riquelme); Facultad de Ciencias Marinas, Universidad Autónoma de Baja California, Ensenada (R. Eaton-González)

Main Article

Figure 3

Distribution plot from droplet digital PCR analysis of Coccidioides spp. fungi DNA from soil samples, Baja California, Mexico. A) Samples from RG; B) samples from RC; C) samples from RLG; and D) samples from AE and Community of San José de la Zorra (CSJZ). The pink line represents the fluorescence threshold. Red text indicates negative sampling sites; green text indicates positive sampling sites. A1–2, negative templates of 28.8 ng of Aspergillus fischeri DNA; AE, Agua Escondida; C+, 0.048 ng of C. posadasii DNA used as positive template; CSJZ, Community of San José de la Zorra; NTC1–3, nontemplate controls of highly refined type I ultrapure (HPLC) water; RC, Rancho Carrizo; RG, Rancho Gilbert; RLG, Rancho las Golondrinas.

Figure 3. Distribution plot from droplet digital PCR analysis of Coccidioides spp. fungi DNA from soil samples, Baja California, Mexico. A) Samples from RG; B) samples from RC; C) samples from RLG; and D) samples from AE and Community of San José de la Zorra (CSJZ). The pink line represents the fluorescence threshold. Red text indicates negative sampling sites; green text indicates positive sampling sites. A1–2, negative templates of 28.8 ng of Aspergillus fischeri DNA; AE, Agua Escondida; C+, 0.048 ng of C. posadasii DNA used as positive template; CSJZ, Community of San José de la Zorra; NTC1–3, nontemplate controls of highly refined type I ultrapure (HPLC) water; RC, Rancho Carrizo; RG, Rancho Gilbert; RLG, Rancho las Golondrinas.

Main Article

Page created: February 20, 2026
Page updated: April 15, 2026
Page reviewed: April 15, 2026
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