Volume 32, Number 4—April 2026
Research
Enhanced Detection of Coccidioides spp. Fungi from Environmental Samples Using Droplet Digital PCR
Jessica Paulette Segovia-Mota, Ricardo Eaton-González, Jimena Carrillo-Tripp, and Meritxell Riquelme
Table
Comparison of nested PCR and ddPCR for detection of Coccidioides spp. fungi from environmental samples, Baja California, Mexico*
| Location | Collection date | No. samples collected | No. (%) nested PCR–positive | No. (%) ddPCR-positive |
|---|---|---|---|---|
| Rancho Gilbert | 2023 Jun 7 | 20 | 5 (25) | 16 (80) |
| Rancho Carrizo | 2024 May 16 | 18 | 0 | 5 (27.8) |
| Rancho Las Golondrinas | 2024 May 16 | 12 | 0 | 1 (8.3) |
| San José de la Zorra | 2024 Jul 2 | 14 | 0 | 5 (35.7) |
| Agua Escondida |
2024 Jul 2 |
12 |
0 |
3 (25) |
| Total | 76 | 5 (6.6) | 30 (39.5) |
*PCR targeted internal transcribe spacer 1 region of Coccidioides. ddPCR, digital droplet PCR.
Page created: February 20, 2026
Page updated: April 15, 2026
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