Disclaimer: Early release articles are not considered as final versions. Any changes will be reflected in the online version in the month the article is officially released.
Volume 32, Number 4—April 2026
Research
Enhanced Detection of Coccidioides spp. Fungi from Environmental Samples Using Droplet Digital PCR
Jessica Paulette Segovia-Mota, Ricardo Eaton-González, Jimena Carrillo-Tripp, and Meritxell Riquelme
Table
Comparison of nested PCR and ddPCR for detection of Coccidioides spp. fungi from environmental samples, Baja California, Mexico*
| Location | Collection date | No. samples collected | No. (%) nested PCR–positive | No. (%) ddPCR-positive |
|---|---|---|---|---|
| Rancho Gilbert | 2023 Jun 7 | 20 | 5 (25) | 16 (80) |
| Rancho Carrizo | 2024 May 16 | 18 | 0 | 5 (27.8) |
| Rancho Las Golondrinas | 2024 May 16 | 12 | 0 | 1 (8.3) |
| San José de la Zorra | 2024 Jul 2 | 14 | 0 | 5 (35.7) |
| Agua Escondida |
2024 Jul 2 |
12 |
0 |
3 (25) |
| Total | 76 | 5 (6.6) | 30 (39.5) |
*PCR targeted internal transcribe spacer 1 region of Coccidioides. ddPCR, digital droplet PCR.
Page created: February 20, 2026
Page updated: March 30, 2026
Page reviewed: March 30, 2026
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.