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Volume 32, Number 9—September 2026

Research

Dual PCR–Sanger Sequencing Assay for Simultaneous sqle-Based Identification and Resistance Detection in Trichophyton Species

Nissrine Abou-ChakraComments to Author , Karin M. Jørgensen, Karen Marie Thyssen Astvad, and Maiken Cavling Arendrup
Author affiliation: Statens Serum Institut, Copenhagen, Denmark (N. Abou-Chakra, K.M. Jørgensen, K.M.T. Astvad, M.C. Arendrup); Rigshospitalet, Copenhagen (M.C. Arendrup); University of Copenhagen, Copenhagen (M.C. Arendrup)

Main Article

Figure 1

Phylogenetic tree of Trichophyton species inferred from sequence variation in the full-length squalene epoxidase (sqle) gene in study of dual PCR–Sanger sequencing assay for simultaneous sqle-based identification and resistance detection in Trichophyton species. Within the T. mentagrophytes species complex, isolates identified as T. mentagrophytes represented ITS genotypes III, III*, IV, and VII. The remaining T. mentagrophytes isolates formed 2 consensus sequences that did not show 100% identity to any sequence in GenBank and were designated DK-I and DK-II. Scale bar indicates number of substitutions per site.

Figure 1. Phylogenetic tree of Trichophyton species inferred from sequence variation in the full-length squalene epoxidase (sqle) gene in study of dual PCR–Sanger sequencing assay for simultaneous sqle-based identification and resistance detection in Trichophyton species. Within the T. mentagrophytes species complex, isolates identified as T. mentagrophytes represented ITS genotypes III, III*, IV, and VII. The remaining T. mentagrophytes isolates formed 2 consensus sequences that did not show 100% identity to any sequence in GenBank and were designated DK-I and DK-II. Scale bar indicates number of substitutions per site.

Main Article

Page created: July 30, 2026
Page updated: August 18, 2026
Page reviewed: August 18, 2026
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