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Volume 32, Number 9—September 2026
Research
Dual PCR–Sanger Sequencing Assay for Simultaneous sqle-Based Identification and Resistance Detection in Trichophyton Species
Nissrine Abou-Chakra
, Karin M. Jørgensen, Karen Marie Thyssen Astvad, and Maiken Cavling Arendrup
, Karin M. Jørgensen, Karen Marie Thyssen Astvad, and Maiken Cavling Arendrup
Figure 2
![Amino acid polymorphisms in squalene epoxidase within the T. mentagrophytes complex in the study of dual PCR-Sanger sequencing assay for simultaneous sqle-based identification and resistance detection in Trichophyton species. Alignment of SQLE amino acid sequences showing lineage-specific patterns: N276/F419 (ITS genotypes III/III*/VII and unassigned genotypes [DK-I–II]), P454 unique to DK-II, and K276/L419 in ITS genotype IV (shared with T. interdigitale and T. indotineae).](/eid/images/26-0819-F2.jpg)
Figure 2. Amino acid polymorphisms in squalene epoxidase within the T. mentagrophytes complex in the study of dual PCR-Sanger sequencing assay for simultaneous sqle-based identification and resistance detection in Trichophyton species. Alignment of SQLE amino acid sequences showing lineage-specific patterns: N276/F419 (ITS genotypes III/III*/VII and unassigned genotypes [DK-I–II]), P454 unique to DK-II, and K276/L419 in ITS genotype IV (shared with T. interdigitale and T. indotineae).
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