Volume 7, Number 6—December 2001
Research
Rapid Identification of Bordetella pertussis Pertactin Gene Variants Using LightCycler Real-Time Polymerase Chain Reaction Combined with Melting Curve Analysis and Gel Electrophoresis
Table 2
Comparison of the results from sequencing and the hybridization probe assay in the determination of the pertactin gene type of Bordetella pertussis strains
Melting temperature |
Melting curve |
||||
---|---|---|---|---|---|
Pertactin allele typea (determined by sequencing) | No. of isolates | Mean | SD | Area | SD |
1 | 9 | 58.78b | 0.26 | 21.64b | 5.64 |
2 | 25 | 56.31 | 0.44 | 1.25 | 0.30 |
3 | 4 | 56.59 | 0.09 | 1.35 | 0.26 |
4 | 5 | 56.57 | 0.29 | 1.43 | 0.25 |
5 | 1 | - | - | - | - |
aOf the strains representing prn1-4, 41 were Finnish clinical isolates, and 2 were Finnish vaccine strains.
bAll P values were <0.0001 when prn1 was compared to prn2, prn3, or prn4.