Volume 7, Number 6—December 2001
Research
Rapid Identification of Bordetella pertussis Pertactin Gene Variants Using LightCycler Real-Time Polymerase Chain Reaction Combined with Melting Curve Analysis and Gel Electrophoresis
Johanna Mäkinen*†
, Matti K. Viljanen*, Jussi Mertsola†, Heikki Arvilommi*, and Qiushui He*
, Matti K. Viljanen*, Jussi Mertsola†, Heikki Arvilommi*, and Qiushui He*
Table 2
Comparison of the results from sequencing and the hybridization probe assay in the determination of the pertactin gene type of Bordetella pertussis strains
| Melting temperature |
Melting curve |
||||
|---|---|---|---|---|---|
| Pertactin allele typea (determined by sequencing) | No. of isolates | Mean | SD | Area | SD |
| 1 | 9 | 58.78b | 0.26 | 21.64b | 5.64 |
| 2 | 25 | 56.31 | 0.44 | 1.25 | 0.30 |
| 3 | 4 | 56.59 | 0.09 | 1.35 | 0.26 |
| 4 | 5 | 56.57 | 0.29 | 1.43 | 0.25 |
| 5 | 1 | - | - | - | - |
aOf the strains representing prn1-4, 41 were Finnish clinical isolates, and 2 were Finnish vaccine strains.
bAll P values were <0.0001 when prn1 was compared to prn2, prn3, or prn4.
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