Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

Volume 7, Number 6—December 2001

Research

Rapid Identification of Bordetella pertussis Pertactin Gene Variants Using LightCycler Real-Time Polymerase Chain Reaction Combined with Melting Curve Analysis and Gel Electrophoresis

Johanna Mäkinen*†Comments to Author , Matti K. Viljanen*, Jussi Mertsola†, Heikki Arvilommi*, and Qiushui He*
Author affiliations: *National Public Health Institute, Department in Turku, Finland;; †Turku University Central Hospital, Turku, Finland

Main Article

Figure 7

Ethidium bromide stained 3% molecular screening agarose gel containing Bordetella pertussis DNA amplified with primers QH8F´ and QH2R. Lanes: 1, negative control including all reagents but no template DNA; 2, 100-bp ladder; 3, B. pertussis strain 1772 of type prn1 (260 bp); 4, Bordetella pertussis clinical isolate of type prn2 (275 bp); 5, B. pertussis clinical isolate of type prn3 (260 bp); 6, B. pertussis clinical isolate of type prn4 (245 bp); and 7, B. pertussis type prn5 (245 bp).

Figure 7. Ethidium bromide stained 3% molecular screening agarose gel containing Bordetella pertussis DNA amplified with primers QH8F´ and QH2R. Lanes: 1, negative control including all reagents but no template DNA; 2, 100-bp ladder; 3, B. pertussis strain 1772 of type prn1 (260 bp); 4, Bordetella pertussis clinical isolate of type prn2 (275 bp); 5, B. pertussis clinical isolate of type prn3 (260 bp); 6, B. pertussis clinical isolate of type prn4 (245 bp); and 7, B. pertussis type prn5 (245 bp).

Main Article

The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.

Ahead Of Print

GO

Past Issues

GO

LATEST PODCAST

Decades of Hepatitis B Vaccination in China— (Length: 11:46)

CDC 24/7 – Saving Lives, Protecting People, Saving Money. Learn More About How CDC Works For You…

Related Information

Additional Resources

CDC 24/7 – Saving Lives, Protecting People, Saving Money. Learn More About How CDC Works For You…

TOP