Volume 7, Number 6—December 2001
Research
Rapid Identification of Bordetella pertussis Pertactin Gene Variants Using LightCycler Real-Time Polymerase Chain Reaction Combined with Melting Curve Analysis and Gel Electrophoresis
Figure 1

Figure 1. Workflow for typing prn alleles. The allele-specific amplification (ASA) assay (step number 3) and the fluorescence resonance energy transfer (FRET) probe assay (step number 4) each require approximately 1 hour.