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Volume 7, Number 6—December 2001

Research

Rapid Identification of Bordetella pertussis Pertactin Gene Variants Using LightCycler Real-Time Polymerase Chain Reaction Combined with Melting Curve Analysis and Gel Electrophoresis

Johanna Mäkinen*†Comments to Author , Matti K. Viljanen*, Jussi Mertsola†, Heikki Arvilommi*, and Qiushui He*
Author affiliations: *National Public Health Institute, Department in Turku, Finland;; †Turku University Central Hospital, Turku, Finland

Main Article

Figure 3

Schematic structure of the prn1 gene, showing the position of the primers used in the allele-specific amplification assay (1.), and the primers and probes used in the fluorescence resonance energy transfer probe assay (2.). Proportions of the gene are not drawn to scale. F = fluorescein; LC = LC red 640; and P = phosphate.

Figure 3. Schematic structure of the prn1 gene, showing the position of the primers used in the allele-specific amplification assay (1.), and the primers and probes used in the fluorescence resonance energy transfer probe assay (2.). Proportions of the gene are not drawn to scale. F = fluorescein; LC = LC red 640; and P = phosphate.

Main Article

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