Volume 10, Number 8—August 2004
Research
Serotype III Streptococcus agalactiae from Bovine Milk and Human Neonatal Infections1
Table 3
Inserted sequences and sequence type (ST) of bovine strains containing the infB A allelea
Inserted sequence site |
|||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
RAPD group | Isolate | Origin | AW-10 | hylB | scpb-lmb | ST | RDP type | ||||||
II |
SH-96-3696 |
Bovine |
3,400b |
None |
None |
67 |
Unknownc |
||||||
III |
NI-96-2836 |
Bovine |
None |
IS1548 |
IS1548 |
19 |
III-2-like |
||||||
SH-96-4807 |
Bovine |
None |
None |
None |
17 |
III-3-like |
|||||||
RF-96-2997 |
Bovine |
None |
None |
None |
94 |
III-1 |
|||||||
IV-A |
1003A |
Human |
None |
IS1548 |
IS1548 |
19 |
III-2 |
||||||
AL-97-0498 SH-96-3417 |
Bovine |
None |
None |
None |
23 |
III-1 |
|||||||
All 17 others |
Bovine |
None |
None |
No productd |
23,90,92 |
III-1-like |
|||||||
IV-C |
NI-96-3213 |
Bovine |
None |
None |
No productd |
23 |
III-1-like |
||||||
ASS-96-666 |
Bovine |
None |
None |
No productd |
23 |
III-1-like |
|||||||
1007B |
Human |
None |
IS1548 |
IS1548/IS1381e |
86 |
III-2 |
|||||||
1009A, 15888 |
Human |
None |
IS1548 |
IS1548 |
19 |
III-2 |
|||||||
13228, 1009B | Human | None | IS1548 (truncated)f | IS1548 | 19 | III-2 |
aRAPD, randomly amplified polymorphic DNA; RDP, restriction digest pattern.
bA 3,400-bp insert is also found in other strains from randomly amplified polymorphic DNA (RAPD) group II (see text and Table 5).
cThis strain appears to be related to the H allele strains described in Table 5.
dNo polymerase chain reaction product was produced from these strains in multiple attempts.
eIn this strain, the insertion sequence IS1381 is upstream from IS1548 in the scpb-lmb locus.
fS1548 in the hylB locus is missing 657 bp from the 3′ end of its open reading frame in this strain.
1Presented in part at the XVth Lancefield International Symposium on Streptococci and Streptococcal Diseases, October 6–11, 2002, Goa, India.