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Volume 13, Number 8—August 2007
Letter

Mycobacterium cosmeticum, Ohio and Venezuela

Robert C. Cooksey*Comments to Author , Jacobus H. de Waard†, Mitchell A. Yakrus*, Sean R. Toney*, Omaira Da Mata†, Scott Nowicki‡, Kevin Sohner‡, Elizabeth Koch‡, Cathy A. Petti§, Roger E. Morey*, and Arjun Srinivasan*
Author affiliations: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA; †Instituto de Biomedicina, Caracas, Venezuela; ‡Ohio Department of Health, Columbus, Ohio, USA; §ARUP Laboratories, Salt Lake City, Utah, USA;

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Figure

Repetitive element (Rep)–PCR (A) and pulsed-field gel electrophoresis (PFGE) (B) patterns of Mycobacterium cosmeticum isolates from 2 patients in Ohio and 1 patient in Venezuela. Rep-PCR was performed by using BOXA1R primer (3), and PFGE was performed with restriction enzyme AseI. Lanes 1, 2, Ohio isolates OH1 and OH2; lanes 3, 4, control strains ATCC BAA-878T and ATCC BAA-879; lane 5, Venezuelan isolate VZ1. DNA size standards are 100-bp (S1) and 48.5-kb marker (S2).

Figure. Repetitive element (Rep)–PCR (A) and pulsed-field gel electrophoresis (PFGE) (B) patterns of Mycobacterium cosmeticum isolates from 2 patients in Ohio and 1 patient in Venezuela. Rep-PCR was performed by using BOXA1R primer (3), and PFGE was performed with restriction enzyme AseI. Lanes 1, 2, Ohio isolates OH1 and OH2; lanes 3, 4, control strains ATCC BAA-878T and ATCC BAA-879; lane 5, Venezuelan isolate VZ1. DNA size standards are 100-bp (S1) and 48.5-kb marker (S2).

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References
  1. Cooksey  RC, de Waard  JH, Yakrus  MA, Rivera  I, Chopite  M, Toney  SR, Mycobacterium cosmeticum sp. nov., a novel rapidly growing species isolated from a cosmetic infection and from a nail salon. Int J Syst Evol Microbiol. 2004;54:238591. DOIPubMedGoogle Scholar
  2. Pfyffer  GE, Brown-Elliott  BA, Wallace  RJ Jr. Mycobacterium: general characteristics, isolation, and staining procedures. In: Murray PR, Baron EJ, Pfaller MA, Jorgensen JH, Yolken RH, editors. Manual of clinical microbiology. Washington: ASM Press; 2003. p. 532–59.
  3. Dombek  PE, Johnson  LK, Zimmerley  ST, Sadowsky  MJ. Use of repetitive DNA sequences and the PCR to differentiate Escherichia coli isolates from human and animal sources. Appl Environ Microbiol. 2000;66:25727. DOIPubMedGoogle Scholar
  4. Wagner  D, Young  LS. Nontuberculous mycobacterial infections: a clinical review. Infection. 2004;32:25770. DOIPubMedGoogle Scholar

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Page updated: June 30, 2010
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The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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