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Volume 15, Number 3—March 2009
Dispatch

Merkel Cell Polyomavirus DNA in Respiratory Specimens from Children and Adults

Seweryn BialasiewiczComments to Author , Stephen B. Lambert, David M. Whiley, Michael D. Nissen, and Theo P. Sloots
Author affiliations: Sir Albert Sakzewski Virus Research Centre, Brisbane, Queensland, Australia (S. Bialasiewicz, S.B. Lambert, D.M. Whiley, M.D. Nissen, T.P. Sloots); University of Queensland Clinical Medical Virology Center, Brisbane (S. Bialasiewicz, S.B. Lambert, D.M. Whiley, M.D. Nissen, T.P. Sloots); Pathology Queensland; Brisbane (M.D. Nissen, T.P. Sloots)

Main Article

Table 2

Nucleotide variation in LT3 and VP1 sequences from 3 MCPyV strains detected in respiratory secretions from patients, Queensland, Australia, 2993*†

Isolate name Nucleotide position and change
LT3
 VP1
843 3825 3875 3909 4022
MCC350‡ (EU375803) G T C C T
MCC339‡ (EU375804) C C G T A
MCV-B1 (LT3: FJ009185, VP1: FJ009188) C C – – –
MCV-B2 (LT3: FJ009186, VP1: FJ009189) C C G – A
MCV-B3 (LT3: FJ009187, VP1: FJ009190) C C – – –

*LT3, partial large T antigen; VP1, major capsid protein; MCPyV, Merkel cell polyomavirus.
†Nucleotide changes are in relation to the prototype strain (boldface). Dashes indicate homology with MCC350. Nucleotide positions are given in a 5′ → 3′ early to late orientation, respective to alignment with MCC350. GenBank accession numbers are shown in parentheses.
‡Original MCPyV strain sequences described by Feng et al. (3).

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References
  1. Allander  T, Andreasson  K, Gupta  S, Bjerkner  A, Bogdanovic  G, Perrsson  MA, Identification of a third human polyomavirus. J Virol. 2007;81:4130–6. DOIPubMedGoogle Scholar
  2. Gaynor  AM, Nissen  MD, Whiley  DM, Mackay  IM, Lambert  SB, Wu  G, Identification of a novel polyomavirus from patients with acute respiratory tract infections. PLoS Pathog. 2007;3:e64. DOIPubMedGoogle Scholar
  3. Feng  H, Shuda  M, Chang  Y, Moore  PS. Clonal integration of a polyomavirus in human Merkel cell carcinoma. Science. 2008;319:1096–100. DOIPubMedGoogle Scholar
  4. Bialasiewicz  S, Whiley  DM, Lambert  SB, Jacob  K, Bletchly  C, Wang  D, Presence of the newly discovered human polyomaviruses KI and WU in Australian patients with acute respiratory tract infection. J Clin Virol. 2008;41:63–8. DOIPubMedGoogle Scholar
  5. Smuts  H, Workman  L. Zar, HJ. Role of human metapneumovirus, human coronavirus NL63 and human bocavirus in infants and young children with acute wheezing. J Med Virol. 2008;80:906–12. DOIPubMedGoogle Scholar
  6. Le  BM, Demertzis  LM, Wu  G, Tibbets  RJ, Buller  R, Arens  MQ, Clinical and epidemiologic characterization of WU polyomavirus infection in St. Louis, USA. Emerg Infect Dis. 2007;13:1936–8.PubMedGoogle Scholar
  7. Garneski  KM, Warcola  AH, Feng  Q, Kiviat  NB, Leonard  JH, Nghiem  P. Merkel cell polyomavirus is more frequently present in North American than Australian Merkel cell carcinoma tumors. J Invest Dermatol. 2009;129:246–8.print.
  8. Ridd  K, Yu  S, Bastian  BC. The presence of polyomavirus in non-melanoma skin cancer in organ transplant recipients is rare. J Invest Dermatol. 2009;129:250–2. DOIPubMedGoogle Scholar
  9. Sloots  TP, Whiley  DM, Lambert  SB, Nissen  MD. Emerging respiratory agents: new viruses for old diseases? J Clin Virol. 2008;42:233–43. DOIPubMedGoogle Scholar
  10. Imperiale  MJ, Major  EO. Polyomaviruses. In: Knipe DM, Griffin DE, Lamb RA, Straus SE, Howley PM, Martin MA, et al., editors. Fields Virology. 5th ed. Vol. 2. Philadelphia: Lippincott Williams & Wilkins, 2007:2268–82.
  11. Reploeg  MD, Storch  GA, Clifford  DB. Bk virus: a clinical review. Clin Infect Dis. 2001;33:191–202. DOIPubMedGoogle Scholar
  12. Dubensky  TW, Villarreal  LP. The primary site of replication alters the eventual site of persistent infection by polyomavirus in mice. J Virol. 1984;50:541–6.PubMedGoogle Scholar

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