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Volume 16, Number 8—August 2010
Research

Bat Coronaviruses and Experimental Infection of Bats, the Philippines

Shumpei Watanabe, Joseph S. Masangkay, Noriyo Nagata, Shigeru Morikawa, Tetsuya Mizutani, Shuetsu Fukushi, Phillip Alviola, Tsutomu Omatsu, Naoya Ueda, Koichiro Iha, Satoshi Taniguchi, Hikaru Fujii, Shumpei Tsuda, Maiko Endoh, Kentaro Kato, Yukinobu Tohya, Shigeru Kyuwa, Yasuhiro Yoshikawa, and Hiroomi AkashiComments to Author 
Author affiliations: University of Tokyo, Tokyo, Japan (S. Watanabe, N. Ueda, K. Iha, S. Taniguchi, H. Fujii, S. Tsuda, M. Endoh, K. Kato, S. Kyuwa, Y. Yoshikawa, H. Akashi); University of the Philippines Los Baños, Laguna, the Philippines (J.S. Masangkay, P. Alviola); National Institute of Infectious Diseases, Tokyo (N. Nagata, S. Morikawa, T. Mizutani, S. Fukushi, T. Omatsu); Nihon University, Kanagawa, Japan (Y. Tohya)

Main Article

Table 2

PCR results for bat coronavirus in fruit bats infected by using bat intestinal samples, the Philippines*

Bat Assay Liver Kidney Lung Spleen Brain Small intestine Large intestine Serum
A RT-PCR – – – – – – + –
qRT-PCR
 ND
 ND
 ND
 ND
 ND
 1.25 × 106
 3.53 × 106
 ND
B RT-PCR – – – – – + + –
qRT-PCR ND ND ND ND ND 1.47 × 106 1.50 ×106 –

*Bat intestinal samples containing 7.8 × 106 copies of coronavirus genome. Values are copies per milligram. RT-PCR, reverse transcription–PCR; –, virus RNA not detected; +, virus RNA detected; qRT-PCR, quantitative RT-PCR; ND, not done.

Main Article

Page created: March 30, 2011
Page updated: March 30, 2011
Page reviewed: March 30, 2011
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