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Volume 14, Number 7—July 2008
Research

Wide Distribution of a High-Virulence Borrelia burgdorferi Clone in Europe and North America

Wei-Gang Qiu*Comments to Author , John F. Bruno†, William D. McCaig*, Yun Xu†, Ian Livey‡, Martin E. Schriefer§, and Benjamin J. Luft†
Author affiliations: *Hunter College of the City University of New York, New York, New York, USA; †Stony Brook University, Stony Brook, New York, USA; ‡Baxter Innovations GmBH, Orth/Donau, Austria; §Centers for Disease Control and Prevention, Fort Collins, Colorado, USA;

Main Article

Table 2

Genomic markers and PCR primers

Locus* Primer sequence (5′ → 3′)† Location‡
BB0057 (gap) F-ATGAAATTGGCTATTAATGG, R-TTGAGCAAGATCAACCACTC Main chromosome (52.5 K)
BB0160 (alr) F-ATGTATAATAATAAAACAATGG, R-ATTTTCTCTTTTCGTATTTTCC Main chromosome (160 K)
BB0243 (glpA) F-ATGGAGGAATATTTAAATTTC, R-GTTCATTTTTCCACTCTTC Main chromosome (249 K)
IGS (rrs-rrlA) 1st round§: F-GGTATGTTTAGTGAGGG, R-GGTTAGAGCGCAGGTCTG; 
2nd round: F-CGTACTGGAAAGTGCGGCTG,
R-GATGTTCAACTCATCCTGGTCCC Main chromosome (444 K)
BB0545 (xylB) F-ATGAATGCTCTTAGTATTG, R-CCCGTTAACAAATAGAC Main chromosome (555 K)
BB0622 (ackA) F-TTGTCAAATACAAAAGG, R-AATGTCTTCAAGAATGG Main chromosome (649 K)
BB0809 (tgt) F-ATGTTTAGTGTAATCAAGAATG, R-ATCGAAATTTTCCTCTTCATAC Main chromosome (855 K)
BBA24 (dpbA) F1-TAATGTTATGATTAAATG, F2-ATGAATAAATATCAAAAAAC, 
R-GAAATTCCAAATAACATC lp54
BBB19 (ospC) F-CCGTTAGTCCAATGGCTCCAG, R-ATGCAAATTAAAGTTAATATC cp26
BBD14 F-ATGATAATAAAAATAAAAAATAATG, R-ATTTTGATTAATTTTAATTTTGCTG lp17

*B31 open reading frame (gene) names. IGS, intergenic spacer.
†F, forward; R, reverse.
‡Approximate starting positions on the B31 genome (29).
§Source (30).

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