Volume 15, Number 2—February 2009
Research
Simian T-Lymphotropic Virus Diversity among Nonhuman Primates, Cameroon
Table 1
Region | Primer set | Forward primer and sequence (5′ → 3′) | Reverse primer and sequence (5′ → 3′) | bp |
---|---|---|---|---|
tax |
Outer | 8699TF1 GTACCCTGTCTACGTTTTCGGCGAT | PGTAXR1 GAIGAYTGIASTACYAAAGATGGCTG | 779 |
Inner | 8699TF2 TTACTGGCCACCTGTCCTGAACAC | PGTAXR2 TTIGGGYAIGGICCGGAAATCAT | 658 | |
Outer | P5TAXF3† CCCTCAAGGTCCTCACCCCGCCGC | P5TAXR3 TAACGGCCAGGTCATTGGAGGTGT | 244 | |
Inner |
P5TAXF2†
AAGTTCCTCCCTCCTTCTTCCATG |
P5TAXR1
TGGTAGAGGTATAAGCACACGATGGTG |
174 |
|
tax-LTR |
Outer | 8699TF6 CATCCGGACCAACTAGGGGCCTTC | PGTATA1+2R1 TCCTGAACYGTCYYYRCGCTTTTATAG | 721 |
Inner | 8699TF7‡ AACAAAAATCCCTACCAAACGCTT | PGTATA1+2R1 TCCTGAACYGTCYYYRCGCTTTTATAG | 695 | |
Inner |
8699TF8§
CAGCCCACCCGCGCACCAGTAATT |
PGTATA1+2R1
TCCTGAACYGTCYYYRCGCTTTTATAG |
589 |
|
LTR | Outer | 8699LF3 CTCTGACGTCTCTCCCTGCCTTGT | PGPBSR1n ATCCCGGACGAGCCCCCA | 612 |
Inner | 8699LF4 CCGGAAAAAACCTTAAACCACCCA | PGPBSR1n ATCCCGGACGAGCCCCCA | 585 |
*bp, base pair; LTR, long terminal repeat; I, inosine; S, G/C; Y, T/C; R, A/G. Nonhuman primates are coded as follows: the first letter of the genus is followed by the first 2 letters of the species name: Cmo, Cercopithecus mona; Cni, C. nictitans. The last 2 letters in the code indicate the study site: AB, Abat.
†Primers used to screen human peripheral blood mononuclear cell DNA for simian T-lymphotropic virus-3 (Cmo8699AB)–like tax sequences.
‡Primer set used for Cni7867AB.
§Primer set used for Cmo8699AB.
1Current affiliation: Stanford University, Stanford, California, USA.
2Current affiliation: University of Pittsburgh Graduate School of Public Health, Pittsburgh, Pennsylvania, USA.
Page created: December 08, 2010
Page updated: December 08, 2010
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