Volume 16, Number 2—February 2010
Dispatch
White-Nose Syndrome Fungus (Geomyces destructans) in Bat, France
Table
Primers used for PCR amplification and sequencing of fungus in bats, France*
Gene | Primer sequence (5′ → 3′) | PCR |
---|---|---|
ITS |
TCCTCCGCTTATTGATATGC | Forward |
GGAAGTAAAAGTCGTAACAAGG |
Reverse |
|
SSU rRNA | CTGGTTGATTCTGCCAGT | Forward |
AAACCTTGTTACGACTTTTA | Reverse | |
CCGGAGAAGGAGCCTGAGAAAC | † | |
AACTTAAAGGAATTGACGGAAG | † | |
CTCATTCCAATTACAAGACC | † | |
GAGTTTCCCCGTGTTGAGTC | † |
*ITS, internal transcribed spacer; SSU, small subunit.
†Used for sequencing only.