Volume 16, Number 4—April 2010
Research
Livestock-associated Methicillin-Resistant Staphylococcus aureus Sequence Type 398 in Humans, Canada
Table 4
Primers used for coverage of the novel SCCmecV subtype in methicillin-resistant Staphylococcus aureus isolates, Canada, 2007–2008*
Primer set | Primer name | Primer 5′ → 3′ | Expected amplicon size, bp | Reference position | SCCmecV found in isolate |
|||||
---|---|---|---|---|---|---|---|---|---|---|
08 BA 02176 | T 49209 | 07 BA 06477 | 08 BA 13895 | 08 BA 08100 | 08 BA 22334 | |||||
1 | OrfX Adpr1 | CATTTAAGATTATGCGTGGAG CATCTGTAAACTGTCCTTTGG | 347 | 443–789 | + | – | + | + | – | + |
2 | RibB2 MecaA1 | TTGTATATGGGGAAACGAAG TGCCAAAATCTCAGGTAAAG | 3623 | 3793–7415 | + | – | + | + | – | + |
3 | MecB1 HypA1 | CTTCACCATTATCGCTTTTAG ACCATTTTTCCCTGGATTAC | 1842 | 9172–11013 | + | + | + | + | + | + |
4 | Hyp3A1 Hyp1B1 | CTTCCACGTATTGGTCTAGC AAGTGAACGCGAAAGATATAG | 2671 | 11636–14306 | + | + | + | + | + | + |
5 | Hyp3B1 CcrCA2 | GCTAGACCAATACGTGGAAG TTTTACCTGAAATGCCTGAG | 3301 | 14287–17587 | + | + | + | + | + | + |
6 | CcrCB1 Hyp6A1 | ATGAAATGGATAGCGAAATG TTGAGTAAGTAGCGGTGTTG | 1330 | 18695–20024 | + | + | + | + | + | + |
7 | Hyp6B1 Crspr1A1 | TGAGCAAGTGATGGAAATG CTTTGAATCCTTTGAAGACG | 2835 | 20331–23165 | + | – | + | + | – | + |
8 | Crspr1B1 Crspr3A1 | AAAAAGTGGTGAGGTTACTTG CTCGTCTATCAATACCACTCG | 711 | 23675–24385 | + | – | + | + | – | + |
9 | Crspr3B1 Crspr7A1 | AACAGATGAACACGGAAAAG TTGGTGGGTATCTCAAAAAG | 2417 | 26166–28582 | + | – | + | + | – | + |
10 | Crspr7B1 Hyp11A1 | GCCTTCTAACGTACCAGTTG TTGCTTCAATGGACTATAAGC | 1511 | 29289–30820 | + | – | + | + | – | + |
11 | Hyp11B1 Hyp12A1 | TTAGGCATGGGGAAATATAG GTCGCAATGTTTTGAAGTG | 1622 | 31373– | + | – | + | + | – | + |
*SCCmec, staphylococcal cassette chromosome mecV subtype; +, positive; –, negative. Testing by PCR.