Volume 16, Number 8—August 2010
Research
White-Nose Syndrome Fungus (Geomyces destructans) in Bats, Europe
Table 2
Fungal culture and PCR results for 23 bats with evidence of fungal colonization tested by light or electron microscopy, Europe*
Country/ location no.† | Sample source | Species | Collection date | No./ hibernacula | PCR result | Culture result | GenBank accession no. |
|
---|---|---|---|---|---|---|---|---|
ITS | SSU rRNA | |||||||
Germany/4 | Hair 2 | Myotis dasycneme | 2008 Feb 25 | 10 | + | + | GU350437 | GU350442 |
Germany/8 | Hair 7 | M. myotis | 2009 Mar 3 | 214 | + | + | GU350436 | GU350441 |
Germany/7 | Tape 8 | M. myotis | 2009 Mar 7 | 57 | + | + | GU999986 | GU999983 |
Hungary/9 | Hair 16 | M. myotis | 2009 Mar 29 | 64 | + | + | GU350434 | GU350439 |
Switzerland/10 | Tape 10 | M. myotis | 2009 Apr 5 | 25 | + | + | GU350433 | GU350438 |
Switzerland/10 | Tape 11 | M. myotis | 2009 Apr 5 | 25 | + | + | GU999984 | GU999981 |
Switzerland/11 | Tape 12 | M. myotis | 2009 Apr 5 | 25 | + | + | GU999985 | GU999982 |
Switzerland/10 | Tape 20 | M. myotis | 2009 Apr 11 | 25 | + | + | GU350435 | GU350440 |
Germany/1 | Hair 1 | M. myotis | 2009 Feb 21 | 65 | + | – | HM222616 | – |
Germany/6 | Hair 20 | M. myotis | 2009 Mar 13 | 100 | + | – | HM222617 | – |
Germany/2 | Tape 1 | M. myotis | 2009 Feb 26 | ≈2,000 | + | – | HM222618 | – |
Germany/2 | Tape 2 | M. myotis | 2009 Feb 26 | ≈2,000 | + | – | HM222619 | – |
Germany/8 | Tape 5 | M. myotis | 2009 Mar 3 | 214 | + | – | HM222620 | – |
Germany/8 | Tape 6 | M. myotis | 2009 Mar 3 | 214 | + | – | HM222621 | – |
Germany/7 | Tape 9 | M. myotis | 2009 Mar 7 | 57 | + | – | HM222622 | – |
Germany/6 | Tape 16 | M. myotis | 2009 Mar 13 | 100 | + | – | HM222623 | – |
Germany/8 | Hair 6 | M. brandtii | 2009 Mar 3 | 214 | + | – | HM222624 | – |
Germany/5 | Hair 3 | M. dasycneme | 2009 Feb 28 | 29 | + | – | HM222625 | – |
Germany/6 | Tape 17 | M. dasycneme | 2009 Mar 13 | 100 | + | – | HM222626 | – |
Germany/3 | Hair 17 | M. daubentonii | 2009 Mar 5 | ≈7,000 | + | – | HM222627 | – |
Hungary/9 | Tape 13 | M. oxygnathus | 2009 Mar 29 | 64 | + | – | HM222628 | – |
United Kingdom/12 | Hair 10 | Rhinolophus ferrumequinum | 2009 Mar 11 | 558 | –‡ | –‡ | HM222629 | – |
United Kingdom/12 | Hair 11 | R. ferrumequinum | 2009 Mar 11 | 558 | –‡ | –‡ | HM222630 | – |
*ITS, internal transcribed spacer; SSU, small subunit; tape, touch imprint with adhesive tape.
†Location number corresponds to a hibernation site in Figure 2.
‡Although samples were negative for G. destructans, they were positive for Penicillium sp. by PCR and culture.