Monitoring and Characterization of Oseltamivir-Resistant Pandemic (H1N1) 2009 Virus, Japan, 2009–2010

Makoto Ujike, Miho Ejima, Akane Anraku, Kozue Shimabukuro, Masatsugu Obuchi, Noriko Kishida, Xu Hong, Emi Takashita, Seiichiro Fujisaki, Kazuyo Yamashita, Hiroshi Horikawa, Yumiko Kato, Akio Oguchi, Nobuyuki Fujita, Masato Tashiro, Takato Odagiri

, and the Influenza Virus Surveillance Group of Japan

Author affiliations: Author affiliations: National Institute of Infectious Diseases, Tokyo, Japan (M. Ujike, M. Ejima, A. Anraku, K. Shimabukuro, M. Obuchi, N. Kishida, X. Hong, E. Takashita, S. Fujisaki, K. Yamashita, M. Tashiro, T. Odagiri); National Institute of Technology and Evaluation, Tokyo (H. Horikawa, Y. Kato, A. Oguchi, N. Fujita)

Main Article

Table A1

Amino acid difference(s) of OR (n = 61) from OS (n = 190) pandemic (H1N1) 2009 virus consensus sequences in neuraminidase protein*

Strains	Amino acid difference(s) of neuraminidase protein
	15	16	34	40	41	43	80	82	83	85	86	95	98	106	138	154	166	195	222†	234	248	257	269	275	286	289	382	386	416	430	452
	M	T	I	L	G	Q	V	S	V	L	A	S	A	I	A	P	V	I	A	V	D	R	M	Y	S	T	G	N	D	R	T
A/Niigata/1459/2009					E‡																			Y/H§
A/Hiroshima/590/2009					E																			Y
A/Shizuoka-C/270/2009					E																			Y
A/Tochigi/471/2009					E																			Y
A/Yokohama/1340/2009					E																			Y
A/Niigata/10016/2009					E							R												Y
A/Aichi/1210/2009								P																Y/H
A/Kobe/92495/2009								P																Y
A/Mie/100/2009								P																Y
A/Saitama/374/2009								P																Y/H
A/Wakayama/318/2009								P																Y/H
A/Yamaguchi/248/2009								P																Y
A/Yamanashi/847/2009								P																Y
A/Yokohama/1563/2009								P																Y
A/Fukuoka/1/2010							M	P																Y
A/Yamagata/29/2010								P					T								N			Y
A/Wakayama-C/1/2010								P							T									Y/H
A/Niigata/10019/2009								P												A				Y
A/Osaka/180/2009																					N			Y
A/Oita/126/2009	I													V							N			Y	G
A/Shizuoka-C/172/2009												G		V							N			Y
A/Osaka/2191/2009																							I	Y
A/Tochigi/609/2009																							I	Y/H
A/Tochigi/612/2009																							I	Y
A/Hokkaido/256/2009																								Y/H					N
A/Shizuoka-C/247/2009																								Y					N
A/Wakayama/291/2009																								Y					N
A/Shiga/45/2009				F																				Y					N
A/Sapporo/576/2009																S							V	Y					N
A/Yokohama/1538/2009																		V						Y/H					N
A/FUKUI/159/2009																								Y			E		N
A/Osaka/2024/2009		I																						Y
A/Shiga/43/2009			V																					Y/H
A/Aichi/1166/2009						K																		Y
A/Sapporo/31/2010									M															Y
A/Shimane/1062/2009										F														Y/H
A/Saitama/396/2009											T													Y
A/Shiga/61/2009																	I							Y
A/Shizuoka/1380/2009																						K		Y
A/Hyogo/2003/2009																						K		Y							I
A/Osaka/2143/2009																								Y		I
A/Mie/137/2009																								Y/H				D
A/Tochigi/373/2009																								Y/H				D
A/Aichi/1019/2009																								Y						Q
A/Chiba/1017/2009																								Y
A/Fukushima/452/2009																								Y
A/Iwate/3/2009																								Y
A/Kitakyusyu/4/2010																								Y
A/Miyazaki/111/2009																								Y
A/Niigata/1233/2009																								Y
A/Niigata/1234/2009																								Y
A/Niigata-C/186/2009																								Y
A/Saitama/408/2009																								Y
A/Saitama-C/88/2009																								Y
A/Sapporo/190/2009																								Y
A/Shimane/188/2009																								Y
A/Tokushima/2/2009																								Y
A/Tottori/225/2009																								Y
A/Yamagata/534/2009																								Y
A/Yamaguchi/22/2009																								Y
A/Yokohama/1394/2009																								Y/H
*OR, oseltamivir resistant; OS, oseltamivir susceptible. †Recent OR seasonal influenza A (H1N1) isolates have R222Q and V234M mutations that would restore viral fitness (23). ‡Of 190 pandemic (H1N1) 2009 virus isolates, G41E, S82P, D248N, M269I, and D416N were observed from 4, 10, 27, 0, and 17 OS pandemic (H1N1) 2009 virus isolates, respectively. §Mixed population of H275 and Y275.

Main Article

¹Members of the Influenza Virus Surveillance Group of Japan are listed at the end of this article.

The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.

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