Figure 5. Seroresponses against trichodysplasia spinulosa–associated polyomavirus (TSV) (A) and BKV polyomavirus (B) for a patient with trichodysplasia spinulosa, the Netherlands. Serial dilutions of serum from a TS patient were tested for reactivity against TSV viral protein 1 (VP1) or BKV VP1 by using the VP1 multiplex antibody-binding assay. Samples were preincubated with soluble recombinant glutathione-S-transferase (GST) (black line), GST-BKV VP1 (red line), or GST-TSV VP1 (blue line). MFI, median fluorescent intensity.