Volume 18, Number 12—December 2012
Research
Transmission Routes for Nipah Virus from Malaysia and Bangladesh
Bronwyn A. Clayton, Deborah Middleton, Jemma Bergfeld, Jessica Haining, Rachel Arkinstall, Linfa Wang, and Glenn A. Marsh
Table 4
NiV in environmental samples after experimental infection of 15 ferrets*
| Virus strain, cage no. (ferret no.) |
log10 NiV copies/mL†
|
||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Dpi 1
|
Dpi 2
|
Dpi 3
|
Dpi 4
|
Dpi 5
|
Dpi 6
|
Dpi 7
|
Dpi 8
|
Dpi 9
|
Dpi 10
|
||||||||||||||||||||
| U | F | U | F | U | F | U | F | U | F | U | F | U | F | U | F | U | F | U | F | ||||||||||
| Bangladesh | |||||||||||||||||||||||||||||
|
1 (B1, B2) |
– | – | – | – | – | – | – | – | – | – | – | – | 5.1‡ | – | NA | NA | NA | NA | NA | NA | |||||||||
|
2 (B3, B4) |
– | – | – | – | – | – | – | – | NS | NS | – | – | 5.2‡ | 4.1 | NA | NA | NA | NA | NA | NA | |||||||||
|
3 (B5, B6) |
– | – | – | – | – | – | – | – | – | – | – | – | 4.1 | 3.4 | 4.6‡ | – | 5.3‡ | 4.6 | NA | NA | |||||||||
|
4 (B7, B8) |
–
|
–
|
|
–
|
–
|
|
–
|
–
|
|
–
|
–
|
|
–
|
–
|
|
–
|
–
|
|
3.6
|
4.3
|
|
–
|
4.9‡
|
|
NS
|
–
|
|
NA
|
NA
|
| Malaysia | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | NA | NA | |||||||||
|
5 (M9) |
– | – | – | – | – | – | – | – | – | – | – | – | 4.9‡ | – | NA | NA | NA | NA | NA | NA | |||||||||
|
6 (M10, 11) |
– | – | – | – | – | 3.6‡ | – | – | – | – | – | – | 4.9‡ | – | NA | NA | NA | NA | NA | NA | |||||||||
|
7 (M12, 13) |
– | – | – | – | – | – | – | – | – | – | 5.1‡ | – | – | – | 4.9‡ | – | – | – | NA | NA | |||||||||
|
8 (M14, 15) |
– | – | – | – | – | – | – | – | – | – | – | – | – | – | 4.6‡ | – | 3.6 | – | – | – | |||||||||
*NiV, Nipah virus; dpi, days postinfection; U, urine; F, feces; –,negative; NA, not applicable because cage was empty after euthanasia of ferrets; NS, no sample available.
†Calculated from standard curve generated for NiV N gene copies by reverse transcription PCR. Samples with mean cycle threshold ≤39.1 (based on duplicate reactions) were defined as NiV positive.
‡Sample was also NiV positive by virus isolation.
Page created: November 20, 2012
Page updated: November 20, 2012
Page reviewed: November 20, 2012
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