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Volume 20, Number 12—December 2014
Dispatch

Novel Amdoparvovirus Infecting Farmed Raccoon Dogs and Arctic Foxes

Xi-Qun ShaoComments to Author , Yong-Jun Wen, Heng-Xing Ba, Xiu-Ting Zhang, Zhi-Gang Yue, Ke-Jian Wang, Chun-Yi Li, Jianming Qiu, and Fu-He YangComments to Author 
Author affiliations: State Key Laboratory for Molecular Biology of Special Economical Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China (X.Q. Shao, Y.J. Wen, H.X. Ba, X.T. Zhang, Z.G. Yue, C.Y. Li, F.H. Yang); State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China (K.J. Wang); University of Kansas Medical Center, Kansas City, Kansas, USA (J Qiu)

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Figure 1

Detection of amdoparvovirus antigens in sick raccoon dogs and infected cells. A) Detection of amdoparvovirus antigens in tissues of sick raccoon dogs. Tissue smears, as described below, were prepared from spleen and kidney samples of sick raccoon dogs and detected with a control mouse serum or anti-AMDV-G serum by using an IFA (8). (a) Mock smear of a spleen tissue from a sick raccoon dog, detected with normal mouse serum as a primary antibody; (b) Control smear of a spleen tissue from a healthy

Figure 1. Detection of amdoparvovirus antigens in sick raccoon dogs and infected cells. A) Detection of amdoparvovirus antigens in tissues of sick raccoon dogs. Tissue smears, as described below, were prepared from spleen and kidney samples of sick raccoon dogs and detected with a control mouse serum or anti-AMDV-G serum by using an IFA (8). (a) Mock smear of a spleen tissue from a sick raccoon dog, detected with normal mouse serum as a primary antibody; (b) Control smear of a spleen tissue from a healthy raccoon dog; (c,d) amdoparvovirus antigen-positive smears of spleen tissue (c) and kidney tissue (d) from a sick raccoon dog, detected with anti-AMDV-G serum. Original magnification ×200. B,C) Detection of amdoparvovirus antigens and virions in infected CrFK cells. B) One milliliter filtered (0.22 μm) pathological spleen samples collected from a sick raccoon dog that had a virus titer of ≈7× 107 vgc/mL, was inoculated to confluent CrFK cells in a T25 flask. Infected cells of the fourth passage were fixed and analyzed with IFA by using anti-AMDV serum (mock- or RFAV-infected CrFK cells). C) Twenty-five milliliter supernatant (≈2× 109 vgc/mL) of RFAV(XQ-JLR)–infected CrFK cell cultures were concentrated. Virions were agglutinated with an anti-RFAV raccoon dog serum and were visualized under a transmission electron microscope (TEM). AMDV, Aleutian mink disease virus; RFAV, raccoon dog and fox amdoparvovirus; IFA, indirect immunofluorescence assay; vgc, viral genome copies. Scale bar = 50 nm.

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