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Volume 20, Number 12—December 2014
Dispatch

Novel Amdoparvovirus Infecting Farmed Raccoon Dogs and Arctic Foxes

Xi-Qun ShaoComments to Author , Yong-Jun Wen, Heng-Xing Ba, Xiu-Ting Zhang, Zhi-Gang Yue, Ke-Jian Wang, Chun-Yi Li, Jianming Qiu, and Fu-He YangComments to Author 
Author affiliations: State Key Laboratory for Molecular Biology of Special Economical Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China (X.Q. Shao, Y.J. Wen, H.X. Ba, X.T. Zhang, Z.G. Yue, C.Y. Li, F.H. Yang); State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China (K.J. Wang); University of Kansas Medical Center, Kansas City, Kansas, USA (J Qiu)

Main Article

Table 1

Oligonucleotide primer pairs used for PCR amplification of amdoparvovirus

Gene, name Primer (5’ → 3’) Ta,† amplicon Use
VP2
AV7-F CCAACAAGTAATGACACCTTGGT 52°C Detection
AV7-R CCTGCTGGTATTATCCATTCAGGA ≈786 bp Sequencing
AV3-F CCAACAAGTAATGACACCTTGGT 53°C Detection
AV3-R GGTTGGTTTGGTTGCTCTCCAAGGA 316 bp
NS1
ANS-F GTAACATGGCTCAGGCTCA 52°C
ANS-R CTCATGCCGAGGTCTCTTGTG 2,008 bp Sequencing
VP1
AVP-F1 CACAAGAGACCTCGGCATGAGTA 52°C
AVP-R1 CCTGCTGGTATTATCCATTCAGGA ≈1,297 bp Sequencing
AVP-F2 GGCTTTGTTCCTTGGAGAGCAAC 52°C
AVP-R2 TGGTAGAATRAGGAAGTACACAK ≈1,443 bp Sequencing

*NS, nonstructural protein; VP, viral structural protein.
†Annealing temperature.

Main Article

Page created: November 19, 2014
Page updated: November 19, 2014
Page reviewed: November 19, 2014
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