Life-Threatening Sochi Virus Infections, Russia
Detlev H. Kruger

, Evgeniy A. Tkachenko, Vyacheslav G. Morozov, Yulia V. Yunicheva, Olga M. Pilikova, Gennadiy Malkin, Aydar A. Ishmukhametov, Patrick Heinemann, Peter T. Witkowski, Boris Klempa, and Tamara K. Dzagurova
Author affiliations: Charité School of Medicine, Berlin, Germany (D.H. Kruger, P. Heinemann, P.T. Witkowski, B. Klempa); Chumakov Institute of Poliomyelitis and Viral Encephalitides, Moscow, Russia (E.A. Tkachenko, G. Malkin, A.A. Ishmukhametov, T.K. Dzagurova); Medical State University, Samara, Russia (V.G. Morozov); Anti-Plague Stations, Sochi, Russia (Y.V. Yunicheva); Anti-Plague Stations, Novorossiysk, Russia (O.M. Pilikova); Slovak Academy of Sciences, Bratislava, Slovakia (B. Klempa)
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Figure 2
Figure 2. Quantification of hantavirus RNA in tissue biopsies from a 50-year-old Sochi virus–infected man (patient no. 59), Russia. Two independent approaches were performed to extract RNA from each organ. Quantitative reverse transcription PCR previously developed for DOBV (7) was used to measure virus load in the analyzed biopsy samples. Three quantitative reverse transcription PCR estimations were conducted for every RNA extraction, followed by calculation of mean values and SDs. Viral RNA levels are shown as genome copies per nanogram of total RNA isolated from the samples. Error bars indicate SD.
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References
Kruger DH,
Figueiredo LTM,
Song JW,
Klempa B.
Hantaviruses—globally emerging pathogens. J Clin Virol.
2015;
64:
128–
36.
DOIPubMed Tkachenko EA,
Okulova NM,
Yunicheva YV,
Morzunov SP,
KhaÄbulina SF,
Riabova TE,
The epizootological and virological characteristics of a natural hantavirus infection focus in the subtropic zone of the Krasnodarsk Territory .
Vopr Virusol.
2005;
50:
14–
9 .
PubMed Klempa B,
Tkachenko EA,
Dzagurova TK,
Yunicheva YV,
Morozov VG,
Okulova NM,
Hemorrhagic fever with renal syndrome caused by 2 lineages of Dobrava hantavirus, Russia. Emerg Infect Dis.
2008;
14:
617–
25.
DOIPubMed Dzagurova TK,
Witkowski PT,
Tkachenko EA,
Klempa B,
Morozov VG,
Auste B,
Isolation of Sochi virus from a fatal case of hantavirus disease with fulminant clinical course. Clin Infect Dis.
2012;
54:
e1–
4.
DOIPubMed Klempa B,
Avsic-Zupanc T,
Clement J,
Dzagurova TK,
Henttonen H,
Heyman P,
Complex evolution and epidemiology of Dobrava-Belgrade hantavirus: definition of genotypes and their characteristics. Arch Virol.
2013;
158:
521–
9.
DOIPubMed Tamura K,
Stecher G,
Peterson D,
Filipski A,
Kumar S.
MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol.
2013;
30:
2725–
9.
DOIPubMed Kramski M,
Meisel H,
Klempa B,
Krüger DH,
Pauli G,
Nitsche A.
Detection and typing of human pathogenic hantaviruses by real-time reverse transcription–PCR and pyrosequencing. Clin Chem.
2007;
53:
1899–
905.
DOIPubMed Okulova NM,
Khliap LA,
Varshavskii AA,
Dzagurova TK,
Iunicheva IV,
Riabova TE,
Spatial structure of natural foci of hantavirus on the territory of northwestern Caucasus .
Zh Mikrobiol Epidemiol Immunobiol.
2013; (
Sep–Oct):
47–
53 .
PubMed Mertz GJ,
Hjelle B,
Crowley M,
Iwamoto G,
Tomicic V,
Vial PA.
Diagnosis and treatment of new world hantavirus infections. Curr Opin Infect Dis.
2006;
19:
437–
42.
DOIPubMed Zhang YZ,
Zou Y,
Fu ZF,
Plyusnin A.
Hantavirus infections in humans and animals, China. Emerg Infect Dis.
2010;
16:
1195–
203.
DOIPubMed Noh JY,
Cheong HJ,
Song JY,
Kim WJ,
Song KJ,
Klein TA,
Clinical and molecular epidemiological features of hemorrhagic fever with renal syndrome in Korea over a 10-year period. J Clin Virol.
2013;
58:
11–
7.
DOIPubMed Avsic-Zupanc T,
Petrovec M,
Furlan P,
Kaps R,
Elgh F,
Lundkvist A.
Hemorrhagic fever with renal syndrome in the Dolenjska region of Slovenia—a 10-year survey. Clin Infect Dis.
1999;
28:
860–
5.
DOIPubMed Papa A,
Antoniadis A.
Hantavirus infections in Greece—an update. Eur J Epidemiol.
2001;
17:
189–
94 .
DOIPubMed Dzagurova TK,
Klempa B,
Tkachenko EA,
Slyusareva GP,
Morozov VG,
Auste B,
Molecular diagnostics of hemorrhagic fever with renal syndrome during a Dobrava virus infection outbreak in the European part of Russia. J Clin Microbiol.
2009;
47:
4029–
36 .
DOIPubMed
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