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Volume 21, Number 12—December 2015

CME ACTIVITY - Dispatch

Life-Threatening Sochi Virus Infections, Russia

Detlev H. KrugerComments to Author , Evgeniy A. Tkachenko, Vyacheslav G. Morozov, Yulia V. Yunicheva, Olga M. Pilikova, Gennadiy Malkin, Aydar A. Ishmukhametov, Patrick Heinemann, Peter T. Witkowski, Boris Klempa, and Tamara K. Dzagurova
Author affiliations: Charité School of Medicine, Berlin, Germany (D.H. Kruger, P. Heinemann, P.T. Witkowski, B. Klempa); Chumakov Institute of Poliomyelitis and Viral Encephalitides, Moscow, Russia (E.A. Tkachenko, G. Malkin, A.A. Ishmukhametov, T.K. Dzagurova); Medical State University, Samara, Russia (V.G. Morozov); Anti-Plague Stations, Sochi, Russia (Y.V. Yunicheva); Anti-Plague Stations, Novorossiysk, Russia (O.M. Pilikova); Slovak Academy of Sciences, Bratislava, Slovakia (B. Klempa)

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Figure 2

Quantification of hantavirus RNA in tissue biopsies from a 50-year-old Sochi virus–infected man (patient no. 59), Russia. Two independent approaches were performed to extract RNA from each organ. Quantitative reverse transcription PCR previously developed for DOBV (7) was used to measure virus load in the analyzed biopsy samples. Three quantitative reverse transcription PCR estimations were conducted for every RNA extraction, followed by calculation of mean values and SDs. Viral RNA levels are s

Figure 2. Quantification of hantavirus RNA in tissue biopsies from a 50-year-old Sochi virus–infected man (patient no. 59), Russia. Two independent approaches were performed to extract RNA from each organ. Quantitative reverse transcription PCR previously developed for DOBV (7) was used to measure virus load in the analyzed biopsy samples. Three quantitative reverse transcription PCR estimations were conducted for every RNA extraction, followed by calculation of mean values and SDs. Viral RNA levels are shown as genome copies per nanogram of total RNA isolated from the samples. Error bars indicate SD.

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