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Volume 22, Number 5—May 2016
Research

Expansion of Shiga Toxin–Producing Escherichia coli by Use of Bovine Antibiotic Growth Promoters

Jong-Chul Kim, Linda Chui, Yang Wang, Jianzhong Shen, and Byeonghwa JeonComments to Author 
Author affiliations: University of Alberta, Edmonton, Alberta, Canada (J.-C. Kim, L. Chui, B. Jeon); Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety at China Agricultural University, Beijing, China (Y. Wang, J. Shen)

Main Article

Figure 5

Transfer of Shiga toxin (Stx) phages by bovine antibiotic growth promoters (bAGPs) in Escherichia coli isolates from cattle. For the confirmation of Stx phage (Stx2Ф) transfer, E. coli O26 (stx2-negative, bovine isolate no. 1 in Figure 3) was used. A) PCR confirmation of the presence or absence of stx2, uspA, eaeA, and rfbEO157 in EDL933 (Stx2Ф donor), O26 before transduction (Stx2Ф recipient), and O26 after transduction (transductant). B) Serotyping of E. coli O157 and O26. +, positive reaction

Figure 5. Transfer of Shiga toxin (Stx) phages by bovine antibiotic growth promoters (bAGPs) in Escherichia coli isolates from cattle. For the confirmation of Stx phage (Stx2Ф) transfer, E. coli O26 (stx2-negative, bovine isolate no. 1 in Figure 3) was used. A) PCR confirmation of the presence or absence of stx2, uspA, eaeA, and rfbEO157 in EDL933 (Stx2Ф donor), O26 before transduction (Stx2Ф recipient), and O26 after transduction (transductant). B) Serotyping of E. coli O157 and O26. +, positive reaction; –, negative reaction. C) Sorbitol fermentation of donor, recipient, and transduced strains on sorbitol MacConkey agar plates. E. coli O157 does not ferment sorbitol, whereas non-O157 E. coli ferments sorbitol and produces pink colonies.

Main Article

Page created: April 13, 2016
Page updated: April 13, 2016
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