Volume 22, Number 5—May 2016
Research
Differences in Genotype, Clinical Features, and Inflammatory Potential of Borrelia burgdorferi sensu stricto Strains from Europe and the United States
Table 1
Genotype | Slovenia, n = 29, no. (%) | United States, n = 90†, no. (%) |
---|---|---|
RST1 | 21 (72) | 38 (42) |
OspC type A | 0 | 27 (30) |
OspC type B‡ | 17 (58) | 11 (12) |
OspC type Q | 2 (7) | 0 |
OspC type R | 1 (3) | 0 |
OspC type M1 | 1 (3) | 0 |
RST2 | 0 | 39 (43) |
OspC type F | 0 | 1 (1) |
OspC type H | 0 | 4 (4) |
OspC type K | 0 | 25 (28) |
OspC type N | 0 | 9 (10) |
RST3 | 8 (28) | 13 (14) |
OspC type D | 0 | 1 (1) |
OspC type E | 0 | 3 (3) |
OspC type G | 0 | 2 (2) |
OspC type I | 0 | 7 (8) |
OspC type L | 7 (24) | 0 |
OspC type S | 1 (3) | 0 |
*RST, ribosomal RNA intergenic sequence type; OspC, outer surface protein C.
†Based on previously published data (10).
‡Found in central Europe and the United States.
References
- Stanek G, Wormser GP, Gray J, Strle F. Lyme borreliosis. Lancet. 2012;379:461–73. DOIPubMedGoogle Scholar
- Wang G, van Dam AP, Schwartz I, Dankert J. Molecular typing of Borrelia burgdorferi sensu lato: taxonomic, epidemiological, and clinical implications. Clin Microbiol Rev. 1999;12:633–53 .PubMedGoogle Scholar
- Jones KL, Muellegger RR, Means TK, Lee M, Glickstein LJ, Damle N, Higher mRNA levels of chemokines and cytokines associated with macrophage activation in erythema migrans skin lesions in patients from the United States than in patients from Austria with Lyme borreliosis. Clin Infect Dis. 2008;46:85–92 . DOIPubMedGoogle Scholar
- Strle F, Nadelman RB, Cimperman J, Nowakowski J, Picken RN, Schwartz I, Comparison of culture-confirmed erythema migrans caused by Borrelia burgdorferi sensu stricto in New York State and by Borrelia afzelii in Slovenia. Ann Intern Med. 1999;130:32–6 . DOIPubMedGoogle Scholar
- Wormser GP, McKenna D, Carlin J, Nadelman RB, Cavaliere LF, Holmgren D, Brief communication: hematogenous dissemination in early Lyme disease. Ann Intern Med. 2005;142:751–5. DOIPubMedGoogle Scholar
- Strle F, Ruzic-Sabljic E, Logar M, Maraspin V, Lotric-Furlan S, Cimperman J, Comparison of erythema migrans caused by Borrelia burgdorferi and Borrelia garinii. Vector Borne Zoonotic Dis. 2011;11:1253–8. DOIPubMedGoogle Scholar
- Strle K, Drouin EE, Shen S, El Khoury J, McHugh G, Ruzic-Sabljic E, Borrelia burgdorferi stimulates macrophages to secrete higher levels of cytokines and chemokines than Borrelia afzelii or Borrelia garinii. J Infect Dis. 2009;200:1936–43. DOIPubMedGoogle Scholar
- Belfaiza J, Postic D, Bellenger E, Baranton G, Girons IS. Genomic fingerprinting of Borrelia burgdorferi sensu lato by pulsed-field gel electrophoresis. J Clin Microbiol. 1993;31:2873–7 .PubMedGoogle Scholar
- Jones KL, Glickstein LJ, Damle N, Sikand VK, McHugh G, Steere AC. Borrelia burgdorferi genetic markers and disseminated disease in patients with early Lyme disease. J Clin Microbiol. 2006;44:4407–13. DOIPubMedGoogle Scholar
- Liveris D, Varde S, Iyer R, Koenig S, Bittker S, Cooper D, Genetic diversity of Borrelia burgdorferi in Lyme disease patients as determined by culture versus direct PCR with clinical specimens. J Clin Microbiol. 1999;37:565–9 .PubMedGoogle Scholar
- Wang IN, Dykhuizen DE, Qiu W, Dunn JJ, Bosler EM, Luft BJ. Genetic diversity of ospC in a local population of Borrelia burgdorferi sensu stricto. Genetics. 1999;151:15–30 .PubMedGoogle Scholar
- Margos G, Gatewood AG, Aanensen DM, Hanincova K, Terekhova D, Vollmer SA, MLST of housekeeping genes captures geographic population structure and suggests a European origin of Borrelia burgdorferi. Proc Natl Acad Sci U S A. 2008;105:8730–5. DOIPubMedGoogle Scholar
- Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Hohna S, MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol. 2012;61:539–42. DOIPubMedGoogle Scholar
- Barbour AG. Isolation and cultivation of Lyme disease spirochetes. Yale J Biol Med. 1984;57:521–5 .PubMedGoogle Scholar
- Strle K, Jones KL, Drouin EE, Li X, Steere AC. Borrelia burgdorferi RST1 (OspC type A) genotype is associated with greater inflammation and more severe Lyme disease. Am J Pathol. 2011;178:2726–39. DOIPubMedGoogle Scholar
- Wormser GP, Brisson D, Liveris D, Hanincova K, Sandigursky S, Nowakowski J, Borrelia burgdorferi genotype predicts the capacity for hematogenous dissemination during early Lyme disease. J Infect Dis. 2008;198:1358–64. DOIPubMedGoogle Scholar
- Wormser GP, Liveris D, Nowakowski J, Nadelman RB, Cavaliere LF, McKenna D, Association of specific subtypes of Borrelia burgdorferi with hematogenous dissemination in early Lyme disease. J Infect Dis. 1999;180:720–5. DOIPubMedGoogle Scholar
- Seifert SN, Khatchikian CE, Zhou W, Brisson D. Evolution and population genomics of the Lyme borreliosis pathogen, Borrelia burgdorferi. Trends Genet. 2015;31:201–7. DOIPubMedGoogle Scholar
- Bennet L, Fraenkel CJ, Garpmo U, Halling A, Ingman M, Ornstein K, Clinical appearance of erythema migrans caused by Borrelia afzelii and Borrelia garinii: effect of the patient’s sex. Wien Klin Wochenschr. 2006;118:531–7. DOIPubMedGoogle Scholar
- Barbour AG, Travinsky B. Evolution and distribution of the ospC gene, a transferable serotype determinant of Borrelia burgdorferi. MBio. 2010;1:e00153–10. DOIPubMedGoogle Scholar
- Rudenko N, Golovchenko M, Honig V, Mallatova N, Krbkova L, Mikulasek P, Detection of Borrelia burgdorferi sensu stricto ospC alleles associated with human Lyme borreliosis worldwide in non-human-biting tick Ixodes affinis and rodent hosts in southeastern United States. Appl Environ Microbiol. 2013;79:1444–53. DOIPubMedGoogle Scholar
- Qiu WG, Bruno JF, McCaig WD, Xu Y, Livey I, Schriefer ME, Wide distribution of a high-virulence Borrelia burgdorferi clone in Europe and North America. Emerg Infect Dis. 2008;14:1097–104. DOIPubMedGoogle Scholar
- Hanincova K, Mukherjee P, Ogden NH, Margos G, Wormser GP, Reed KD, Multilocus sequence typing of Borrelia burgdorferi suggests existence of lineages with differential pathogenic properties in humans. PLoS ONE. 2013;8:e73066 . DOIPubMedGoogle Scholar
- Jungnick S, Margos G, Rieger M, Dzaferovic E, Bent SJ, Overzier E, Borrelia burgdorferi sensu stricto and Borrelia afzelii: population structure and differential pathogenicity. Int J Med Microbiol. 2015;305:673–81. DOIPubMedGoogle Scholar
- Sadziene A, Wilske B, Ferdows MS, Barbour AG. The cryptic ospC gene of Borrelia burgdorferi B31 is located on a circular plasmid. Infect Immun. 1993;61:2192–5 .PubMedGoogle Scholar
- Carlsson SA, Granlund H, Jansson C, Nyman D, Wahlberg P. Characteristics of erythema migrans in Borrelia afzelii and Borrelia garinii infections. Scand J Infect Dis. 2003;35:31–3. DOIPubMedGoogle Scholar
- Logar M, Ruzic-Sabljic E, Maraspin V, Lotric-Furlan S, Cimperman J, Jurca T, Comparison of erythema migrans caused by Borrelia afzelii and Borrelia garinii. Infection. 2004;32:15–9. DOIPubMedGoogle Scholar
- Strle F, Ruzic-Sabljic E, Logar M, Maraspin V, Lotric-Furlan S, Cimperman J, Comparison of erythema migrans caused by Borrelia burgdorferi and Borrelia garinii. Vector Borne Zoonotic Dis. 2011;11:1253–8. DOIPubMedGoogle Scholar
- Strle F, Ruzic-Sabljic E, Cimperman J, Lotric-Furlan S, Maraspin V. Comparison of findings for patients with Borrelia garinii and Borrelia afzelii isolated from cerebrospinal fluid. Clin Infect Dis. 2006;43:704–10. DOIPubMedGoogle Scholar
- Mason LM, Herkes EA, Krupna-Gaylord MA, Oei A, Van der Poll T, Wormser GP, Borrelia burgdorferi clinical isolates induce human innate immune responses that are not dependent on genotype. Immunobiology. 2015;220:1141–50. DOIPubMedGoogle Scholar
- Strle K, Stupica D, Drouin EE, Steere AC, Strle F. Elevated levels of IL-23 in a subset of patients with post-Lyme disease symptoms following erythema migrans. Clin Infect Dis. 2014;58:372–80. DOIPubMedGoogle Scholar
1These authors contributed equally to this article.
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