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Volume 23, Number 2—February 2017
Research

Highly Pathogenic Influenza A(H5Nx) Viruses with Altered H5 Receptor-Binding Specificity

Hongbo Guo1, Erik de Vries1, Ryan McBride, Jojanneke Dekkers, Wenjie Peng, Kim M. Bouwman, Corwin Nycholat, M. Helene Verheije, James C. Paulson, Frank J.M. van Kuppeveld, and Cornelis A.M. de HaanComments to Author 
Author affiliations: Utrecht University, Utrecht, the Netherlands (H. Guo, E. de Vries, J. Dekkers, K.M. Bouwman, M.H. Verheije, F.J.M. van Kuppeveld, C.A.M. de Haan); The Scripps Research Institute, La Jolla, California, USA (R. McBride, W. Peng, C. Nycholat, J.C. Paulson)

Main Article

Figure 4

Analysis of binding of influenza A virus H5N12.3.4 and H5N8 hemagglutinins to sialylated glycans by using biolayer interferometry. A) 3′SLN, B) 3′SLeX, C) 3′SLNLN, D) 6′SLNLN. After complexing biotinylated glycans with streptavidin sensors, association and subsequent dissociation of H5 proteins complexed with StrepMAB-classic (IBA GmbH, Göttingen, Germany) was determined. Blue squares, N-acetylglucosamine; yellow circles, galactose; purple diamonds, sialic acid; red triangles, fucose. The dotted

Figure 4. Analysis of binding of influenza A virus H5N12.3.4 and H5N8 hemagglutinins to sialylated glycans by using biolayer interferometry. A) 3′SLN, B) 3′SLeX, C) 3′SLNLN, D) 6′SLNLN. After complexing biotinylated glycans with streptavidin sensors, association and subsequent dissociation of H5 proteins complexed with StrepMAB-classic (IBA GmbH, Göttingen, Germany) was determined. Blue squares, N-acetylglucosamine; yellow circles, galactose; purple diamonds, sialic acid; red triangles, fucose. The dotted lines at the 20-min time points distinguish the association and dissociation phases. H5N12.3.4, novel H5N1 virus clade 2.3.4.

Main Article

1These authors contributed equally to this article.

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